Supercritical fluid chromatography/orbitrap mass spectrometry based lipidomics platform coupled with automated lipid identification software for accurate lipid profiling

Takayuki Yamada, Takato Uchikata, Shigeru Sakamoto, Yasuto Yokoi, Shin Nishiumi, Masaru Yoshida, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

We developed a practical analytical system for high-throughput and comprehensive lipid profiling using a supercritical fluid chromatography (SFC) system coupled to an Orbitrap Fourier transform mass spectrometer (Orbitrap FT-MS). Using our SFC method, polar lipid molecular species were separated based on not only their fatty acyl moieties but also their polar head groups, using a single octadecylsilyl (ODS) column. In addition, because automatic data processing software was used for the identification of lipid molecular species, the analysis time including data processing was about a half an hour per sample. A variety of lipid molecular species were detected in mouse plasma, and isomers which often co-elute in reverse phase separation were identified accurately and quantified individually. To the best of our knowledge, this is the first report describing the chromatographic separation of lipids based on both fatty acyl moieties and polar head groups, using a single ODS column. Our results demonstrate that SFC/MS is a powerful tool for the simultaneous analysis of diverse lipid molecular species.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalJournal of Chromatography A
Volume1301
DOIs
Publication statusPublished - Aug 2 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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