Supercritical fluid extraction as a preparation method for mass spectrometry of dried blood spots

Atsuki Matsubara, Yoshihiro Izumi, Shin Nishiumi, Makoto Suzuki, Takeshi Azuma, Eiichiro Fukusaki, Takeshi Bamba, Masaru Yoshida

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The potential of supercritical fluid extraction (SFE) as a preparation method for mass spectrometry of dried blood spots (DBS) was examined. SFE is generally used for the extraction of hydrophobic compounds, but hydrophilic metabolites such as amino acids, amines, and nucleic-acid-related metabolites could be extracted by adding a low level of methanol as a modifier. Under the optimized conditions, over 200 metabolites were detected from a dried serum spot, of which over 160 metabolites could be analyzed stably (RSD <20%). These results show that SFE is an effective extraction method of metabolites with a wide range of polarity in DBS.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume969
DOIs
Publication statusPublished - Oct 15 2014
Externally publishedYes

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Supercritical Fluid Chromatography
Supercritical fluid extraction
Metabolites
Mass spectrometry
Mass Spectrometry
Blood
Supercritical fluids
Nucleic Acids
Amines
Methanol
Amino Acids
Serum

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

Supercritical fluid extraction as a preparation method for mass spectrometry of dried blood spots. / Matsubara, Atsuki; Izumi, Yoshihiro; Nishiumi, Shin; Suzuki, Makoto; Azuma, Takeshi; Fukusaki, Eiichiro; Bamba, Takeshi; Yoshida, Masaru.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 969, 15.10.2014, p. 199-204.

Research output: Contribution to journalArticle

Matsubara, Atsuki ; Izumi, Yoshihiro ; Nishiumi, Shin ; Suzuki, Makoto ; Azuma, Takeshi ; Fukusaki, Eiichiro ; Bamba, Takeshi ; Yoshida, Masaru. / Supercritical fluid extraction as a preparation method for mass spectrometry of dried blood spots. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2014 ; Vol. 969. pp. 199-204.
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