Superoxide anion impairs Ca2+ mobilization in cultured human nasal epithelial cells

Tetsuya Koyama, Masahiro Oike, Sohtaro Komiyama, Yushi Ito

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We examined the effects of superoxide anion (O2/-) on the intracellular Ca2+ concentration in cultured human nasal epithelial cells. The cells were exposed to O2/- by pretreatment with xanthine (X) and xanthine oxidase (XO); control cells were treated with X alone. When Ca2+-containing Krebs solution was reperfused in the thapsigargin-treated, store- depleted cells, reapplication-induced intracellular Ca2+ concentration elevation was significantly smaller in X/XO-treated cells than in the control cells, suggesting that O2/- impairs Ca2+ release-activated Ca2+ entry (CRAC). Bath application of ATP induced a steep Ca2+ transient in both control and X/XO- treated cells. However, the concentration-response curve of the ATP-induced Ca2+ transient was shifted to a higher concentration in X/XO-treated cells. The impairments of CRAC and ATP-induced Ca2+ transient induced by X/XO were reversed by superoxide dismutase. Furthermore, all these X/XO-induced effects were also observed in cells pretreated with pyrogallol, also an O2/- donor. These results indicate that O2/- impairs at least two mechanisms involved in Ca2+ mobilization in human nasal epithelial cells, i.e., CRAC and ATP-induced Ca2+ release.

Original languageEnglish
Pages (from-to)L1089-L1095
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume277
Issue number6 21-6
Publication statusPublished - Dec 1 1999

Fingerprint

Nose
Superoxides
Xanthine Oxidase
Epithelial Cells
Adenosine Triphosphate
Pyrogallol
Xanthine
Thapsigargin
Baths
Superoxide Dismutase

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Superoxide anion impairs Ca2+ mobilization in cultured human nasal epithelial cells. / Koyama, Tetsuya; Oike, Masahiro; Komiyama, Sohtaro; Ito, Yushi.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 277, No. 6 21-6, 01.12.1999, p. L1089-L1095.

Research output: Contribution to journalArticle

@article{d48966d5bca84950a43402d2e54697f9,
title = "Superoxide anion impairs Ca2+ mobilization in cultured human nasal epithelial cells",
abstract = "We examined the effects of superoxide anion (O2/-) on the intracellular Ca2+ concentration in cultured human nasal epithelial cells. The cells were exposed to O2/- by pretreatment with xanthine (X) and xanthine oxidase (XO); control cells were treated with X alone. When Ca2+-containing Krebs solution was reperfused in the thapsigargin-treated, store- depleted cells, reapplication-induced intracellular Ca2+ concentration elevation was significantly smaller in X/XO-treated cells than in the control cells, suggesting that O2/- impairs Ca2+ release-activated Ca2+ entry (CRAC). Bath application of ATP induced a steep Ca2+ transient in both control and X/XO- treated cells. However, the concentration-response curve of the ATP-induced Ca2+ transient was shifted to a higher concentration in X/XO-treated cells. The impairments of CRAC and ATP-induced Ca2+ transient induced by X/XO were reversed by superoxide dismutase. Furthermore, all these X/XO-induced effects were also observed in cells pretreated with pyrogallol, also an O2/- donor. These results indicate that O2/- impairs at least two mechanisms involved in Ca2+ mobilization in human nasal epithelial cells, i.e., CRAC and ATP-induced Ca2+ release.",
author = "Tetsuya Koyama and Masahiro Oike and Sohtaro Komiyama and Yushi Ito",
year = "1999",
month = "12",
day = "1",
language = "English",
volume = "277",
pages = "L1089--L1095",
journal = "American Journal of Physiology",
issn = "1040-0605",
publisher = "American Physiological Society",
number = "6 21-6",

}

TY - JOUR

T1 - Superoxide anion impairs Ca2+ mobilization in cultured human nasal epithelial cells

AU - Koyama, Tetsuya

AU - Oike, Masahiro

AU - Komiyama, Sohtaro

AU - Ito, Yushi

PY - 1999/12/1

Y1 - 1999/12/1

N2 - We examined the effects of superoxide anion (O2/-) on the intracellular Ca2+ concentration in cultured human nasal epithelial cells. The cells were exposed to O2/- by pretreatment with xanthine (X) and xanthine oxidase (XO); control cells were treated with X alone. When Ca2+-containing Krebs solution was reperfused in the thapsigargin-treated, store- depleted cells, reapplication-induced intracellular Ca2+ concentration elevation was significantly smaller in X/XO-treated cells than in the control cells, suggesting that O2/- impairs Ca2+ release-activated Ca2+ entry (CRAC). Bath application of ATP induced a steep Ca2+ transient in both control and X/XO- treated cells. However, the concentration-response curve of the ATP-induced Ca2+ transient was shifted to a higher concentration in X/XO-treated cells. The impairments of CRAC and ATP-induced Ca2+ transient induced by X/XO were reversed by superoxide dismutase. Furthermore, all these X/XO-induced effects were also observed in cells pretreated with pyrogallol, also an O2/- donor. These results indicate that O2/- impairs at least two mechanisms involved in Ca2+ mobilization in human nasal epithelial cells, i.e., CRAC and ATP-induced Ca2+ release.

AB - We examined the effects of superoxide anion (O2/-) on the intracellular Ca2+ concentration in cultured human nasal epithelial cells. The cells were exposed to O2/- by pretreatment with xanthine (X) and xanthine oxidase (XO); control cells were treated with X alone. When Ca2+-containing Krebs solution was reperfused in the thapsigargin-treated, store- depleted cells, reapplication-induced intracellular Ca2+ concentration elevation was significantly smaller in X/XO-treated cells than in the control cells, suggesting that O2/- impairs Ca2+ release-activated Ca2+ entry (CRAC). Bath application of ATP induced a steep Ca2+ transient in both control and X/XO- treated cells. However, the concentration-response curve of the ATP-induced Ca2+ transient was shifted to a higher concentration in X/XO-treated cells. The impairments of CRAC and ATP-induced Ca2+ transient induced by X/XO were reversed by superoxide dismutase. Furthermore, all these X/XO-induced effects were also observed in cells pretreated with pyrogallol, also an O2/- donor. These results indicate that O2/- impairs at least two mechanisms involved in Ca2+ mobilization in human nasal epithelial cells, i.e., CRAC and ATP-induced Ca2+ release.

UR - http://www.scopus.com/inward/record.url?scp=0033379843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033379843&partnerID=8YFLogxK

M3 - Article

C2 - 10600877

AN - SCOPUS:0033379843

VL - 277

SP - L1089-L1095

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1040-0605

IS - 6 21-6

ER -