Superoxide anion impairs contractility in cultured aortic smooth muscle cells

Chiwaka Kimura, Wei Cheng, Kazunari Hisadome, Yi Ping Wang, Tetsuya Koyama, Yuji Karashima, Masahiro Oike, Yushi Ito

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Abstract

We examined the effects of superoxide anion (O2-) generated by xanthine plus xanthine oxidase (X/XO) on the intracellular Ca2+ concentration ([Ca2+]i) and muscle contractility in cultured bovine aortic smooth muscle cells (BASMC). Cells were grown on collagen-coated dish for the measurement of [Ca2+]i. Pretreatment with X/XO inhibited ATP-induced Ca2+ transient and Ca2+ release-activated Ca2+ entry (CRAC) after thapsigargin-induced store depletion, both of which were reversed by superoxide dismutase (SOD). In contrast, Ca2+ transients induced by high-K+ solution and Ca2+ ionophore A-23187 were not affected by X/XO. BASMC-embedded collagen gel lattice, which was pretreated with xanthine alone, showed contraction in response to ATP, thapsigargin, high-K+ solution, and A-23187. Pretreatment of the gel with X/XO impaired gel contraction not only by ATP and thapsigargin, but also by high-K+ solution and A-23187. The X/XO-treated gel showed normal contraction; however, when SOD was present during the pretreatment period. These results indicate that O2- attenuates smooth muscle contraction by impairing CRAC, ATP-induced Ca2+ transient, and Ca2+ sensitivity in BASMC.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume283
Issue number1 52-1
Publication statusPublished - Jul 10 2002

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Xanthine
Xanthine Oxidase
Superoxides
Smooth Muscle Myocytes
Thapsigargin
Calcimycin
Adenosine Triphosphate
Gels
Superoxide Dismutase
Collagen
Ionophores
Muscle Contraction
Smooth Muscle
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Superoxide anion impairs contractility in cultured aortic smooth muscle cells. / Kimura, Chiwaka; Cheng, Wei; Hisadome, Kazunari; Wang, Yi Ping; Koyama, Tetsuya; Karashima, Yuji; Oike, Masahiro; Ito, Yushi.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 283, No. 1 52-1, 10.07.2002.

Research output: Contribution to journalArticle

Kimura, Chiwaka ; Cheng, Wei ; Hisadome, Kazunari ; Wang, Yi Ping ; Koyama, Tetsuya ; Karashima, Yuji ; Oike, Masahiro ; Ito, Yushi. / Superoxide anion impairs contractility in cultured aortic smooth muscle cells. In: American Journal of Physiology - Heart and Circulatory Physiology. 2002 ; Vol. 283, No. 1 52-1.
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