Superoxide dismutase from the silkworm, Bombyx mori: Sequence, distribution, and overexpression

Kohji Yamamoto, Pingbo Zhang, Yutaka Banno, Hiroshi Fujii, Fumio Miake, Nobuhiro Kashige, Yoichi Aso

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Superoxide dismutase (SOD) is an enzyme facilitating the removal of superoxide anions from living organisms. This study focused on SOD from the silkworm, Bombyx mori (bmSOD). cDNA encoding bmSOD was amplified by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequence of bmSOD indicated that the residues forming the Cu/Zn binding site are conserved and that the sequence is in 60% identity to that of the Drosophlla melanogaster. B. mori SOD was also close to the D. melanogaster SOD in a phylogenetic tree. The bmSOD mRNA and the enzyme activity were widely distributed in diverse tissues. bmSOD functionally overexpressed in Escherichia coli in a soluble form was purified, and its stability was examined. bmSOD at 4°C retained almost all of its original activity after incubation at pH 4-11 for 24 h. Incubation (pH 7) for 30 min at temperatures below 40°C also affected activity insignificantly.

Original languageEnglish
Pages (from-to)507-514
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number3
DOIs
Publication statusPublished - Mar 1 2005

Fingerprint

Bombyx
Superoxide Dismutase
Conserved Sequence
Polymerase chain reaction
RNA-Directed DNA Polymerase
Enzyme activity
Enzymes
Reverse Transcriptase Polymerase Chain Reaction
Superoxides
Escherichia coli
Amino Acid Sequence
Complementary DNA
Binding Sites
Tissue
Amino Acids
Messenger RNA
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Superoxide dismutase from the silkworm, Bombyx mori : Sequence, distribution, and overexpression. / Yamamoto, Kohji; Zhang, Pingbo; Banno, Yutaka; Fujii, Hiroshi; Miake, Fumio; Kashige, Nobuhiro; Aso, Yoichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 3, 01.03.2005, p. 507-514.

Research output: Contribution to journalArticle

Yamamoto, Kohji ; Zhang, Pingbo ; Banno, Yutaka ; Fujii, Hiroshi ; Miake, Fumio ; Kashige, Nobuhiro ; Aso, Yoichi. / Superoxide dismutase from the silkworm, Bombyx mori : Sequence, distribution, and overexpression. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 3. pp. 507-514.
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