Superoxide production of human polymorphonuclear leukocytes stimulated by leukotriene B4

Hideki Sumimoto, Koichiro Takeshige, Shigeki Minakami

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Leukotriene B4 stimulated a transient production of superoxide anions (O-2) by human polymorphonuclear leukocytes which continued for only about 1 min. The production was dependent on Ca2+ in the suspending medium and no production was observed without the addition of calcium. The concentrations of leukotriene B4 and calcium for the half-maximal production were about 1 μM and 200 μM, respectively. 8-(N,N,-Diethylamino)-octyl-3,4,5-trimethoxybenzoate (TMB-8), an intracellular calcium antagonist, did not inhibit the O-2 production stimulated by leukotriene B4 in the presence of calcium, while N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin inhibitor, did. When leukotriene B4 was added to the cells treated with cytochalasin B, the production of O-2 was biphasic: an initial rapid phase, followed by a slow one. The slow phase was also dependent on Ca2+ concentrations but it could be induced even without the addition of Ca2+ to the medium. The cells treated with both cytochalasin B and TMB-8 in Ca2+-free medium showed a negligible production of superoxide on addition of leukotriene B4, but the production appeared upon addition of CaCl2. These findings suggest that the superoxide production stimulated by leukotriene B4 is associated with the influx of Ca2+.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalBBA - Molecular Cell Research
Volume803
Issue number4
DOIs
Publication statusPublished - Apr 16 1984

Fingerprint

Leukotriene B4
Superoxides
Neutrophils
Calcium
Cytochalasin B
Calmodulin
8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Superoxide production of human polymorphonuclear leukocytes stimulated by leukotriene B4. / Sumimoto, Hideki; Takeshige, Koichiro; Minakami, Shigeki.

In: BBA - Molecular Cell Research, Vol. 803, No. 4, 16.04.1984, p. 271-277.

Research output: Contribution to journalArticle

Sumimoto, Hideki ; Takeshige, Koichiro ; Minakami, Shigeki. / Superoxide production of human polymorphonuclear leukocytes stimulated by leukotriene B4. In: BBA - Molecular Cell Research. 1984 ; Vol. 803, No. 4. pp. 271-277.
@article{5aac599d402344569158f9f2fde62e7b,
title = "Superoxide production of human polymorphonuclear leukocytes stimulated by leukotriene B4",
abstract = "Leukotriene B4 stimulated a transient production of superoxide anions (O-2) by human polymorphonuclear leukocytes which continued for only about 1 min. The production was dependent on Ca2+ in the suspending medium and no production was observed without the addition of calcium. The concentrations of leukotriene B4 and calcium for the half-maximal production were about 1 μM and 200 μM, respectively. 8-(N,N,-Diethylamino)-octyl-3,4,5-trimethoxybenzoate (TMB-8), an intracellular calcium antagonist, did not inhibit the O-2 production stimulated by leukotriene B4 in the presence of calcium, while N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin inhibitor, did. When leukotriene B4 was added to the cells treated with cytochalasin B, the production of O-2 was biphasic: an initial rapid phase, followed by a slow one. The slow phase was also dependent on Ca2+ concentrations but it could be induced even without the addition of Ca2+ to the medium. The cells treated with both cytochalasin B and TMB-8 in Ca2+-free medium showed a negligible production of superoxide on addition of leukotriene B4, but the production appeared upon addition of CaCl2. These findings suggest that the superoxide production stimulated by leukotriene B4 is associated with the influx of Ca2+.",
author = "Hideki Sumimoto and Koichiro Takeshige and Shigeki Minakami",
year = "1984",
month = "4",
day = "16",
doi = "10.1016/0167-4889(84)90117-4",
language = "English",
volume = "803",
pages = "271--277",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Superoxide production of human polymorphonuclear leukocytes stimulated by leukotriene B4

AU - Sumimoto, Hideki

AU - Takeshige, Koichiro

AU - Minakami, Shigeki

PY - 1984/4/16

Y1 - 1984/4/16

N2 - Leukotriene B4 stimulated a transient production of superoxide anions (O-2) by human polymorphonuclear leukocytes which continued for only about 1 min. The production was dependent on Ca2+ in the suspending medium and no production was observed without the addition of calcium. The concentrations of leukotriene B4 and calcium for the half-maximal production were about 1 μM and 200 μM, respectively. 8-(N,N,-Diethylamino)-octyl-3,4,5-trimethoxybenzoate (TMB-8), an intracellular calcium antagonist, did not inhibit the O-2 production stimulated by leukotriene B4 in the presence of calcium, while N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin inhibitor, did. When leukotriene B4 was added to the cells treated with cytochalasin B, the production of O-2 was biphasic: an initial rapid phase, followed by a slow one. The slow phase was also dependent on Ca2+ concentrations but it could be induced even without the addition of Ca2+ to the medium. The cells treated with both cytochalasin B and TMB-8 in Ca2+-free medium showed a negligible production of superoxide on addition of leukotriene B4, but the production appeared upon addition of CaCl2. These findings suggest that the superoxide production stimulated by leukotriene B4 is associated with the influx of Ca2+.

AB - Leukotriene B4 stimulated a transient production of superoxide anions (O-2) by human polymorphonuclear leukocytes which continued for only about 1 min. The production was dependent on Ca2+ in the suspending medium and no production was observed without the addition of calcium. The concentrations of leukotriene B4 and calcium for the half-maximal production were about 1 μM and 200 μM, respectively. 8-(N,N,-Diethylamino)-octyl-3,4,5-trimethoxybenzoate (TMB-8), an intracellular calcium antagonist, did not inhibit the O-2 production stimulated by leukotriene B4 in the presence of calcium, while N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin inhibitor, did. When leukotriene B4 was added to the cells treated with cytochalasin B, the production of O-2 was biphasic: an initial rapid phase, followed by a slow one. The slow phase was also dependent on Ca2+ concentrations but it could be induced even without the addition of Ca2+ to the medium. The cells treated with both cytochalasin B and TMB-8 in Ca2+-free medium showed a negligible production of superoxide on addition of leukotriene B4, but the production appeared upon addition of CaCl2. These findings suggest that the superoxide production stimulated by leukotriene B4 is associated with the influx of Ca2+.

UR - http://www.scopus.com/inward/record.url?scp=0021321871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021321871&partnerID=8YFLogxK

U2 - 10.1016/0167-4889(84)90117-4

DO - 10.1016/0167-4889(84)90117-4

M3 - Article

VL - 803

SP - 271

EP - 277

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 4

ER -