Supplementary effect of hydrogen peroxide as a pre-disinfectant for sterilizing rhizome bud explants of Zantedeschia aethiopica L. With Chlorine Dioxide

Li Ru Chen, Tung Chuan Hsiung, Kuan Hung Lin, Tzu Bin Huang, Meng Yuan Huang, Akira Wakana

Research output: Contribution to journalArticle

Abstract

Zantedeschia spp. (calla lily) are ornamental tuberous/rhizomatous plants of economic importance worldwide. In vitro micropropagation techniques have been widely used to mass-produce virus-free calla lilies. However, effective removal of contaminants is a major problem for the in vitro establishment of the calla lily propagation. In this study, we applied hydrogen peroxide (HjOJ as a pre-disinfectant for sterilizing rhizome bud explants of Z. aethiopica followed by sterilization with sodium hypochlorite (NaOCl) or chlorine dioxide (ClO2) to eliminate in vitro contamination. Results showed that application of 5% (w/v) H2O2 for 5minutes followed by 1% (w/v) NaOCl or 60-180 mg-L"1 ClO2 for 15minutes significantly reduced the contamination rate to < 38% while maintaining the vigor of explant tissues. Surviving explants sterilized with H2O2 followed by ClO2 exhibited a higher rate (>75%) of those showing shoot development than those sterilized with NaOCl (54%). The two-step sterilization method with H2O2 and ClO2 synergistically optimized the disinfection efficiency and explants viability.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume62
Issue number1
Publication statusPublished - Feb 2017

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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