Abstract
Regenerative intramuscular motor-innervation is thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies showed that resident myogenic stem cells, satellite cells, up-regulate a secreted neural-chemorepellent semaphorin 3A (Sema3A) during the early-differentiation period, in response to hepatocyte growth factor (HGF) elevated in injured muscle. However, a paracrine source of the HGF release is still unknown. Very recently, we proposed a possible contribution of anti-inflammatory macrophages (CD206-positive M2) by showing that M2 cells infiltrate predominantly at the early-differentiation phase (3-5 days post-injury) and produce/secrete large amounts of HGF. However, in understanding this concept there still remains a critical need to examine if phagocytotic pro-inflammatory macrophages (CD86-positive M1), another activated-phenotype still present at the early-differentiation phase concerned, produce HGF upon muscle injury. The current immunocytochemical study demonstrated that the HGF expression is negative for M1 prepared from cardiotoxin-injured Tibialis anterior muscle at day 5, in contrast to the intense fluorescent-signal of M2 served as a positive control. This supplementary result advances our understanding of a spatiotemporal burst of HGF secretion from M2 populations (not M1) to impact Sema3A expression, which ensures a coordinated delay in attachment of motoneuron terminals onto damaged and generating fibers during the early phase of muscle regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 994-1000 |
| Number of pages | 7 |
| Journal | Animal science journal = Nihon chikusan Gakkaihō |
| Volume | 85 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 1 2014 |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)
Cite this
Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration. / Sawano, Shoko; Suzuki, Takahiro; Do, Mai Khoi Q.; Ohtsubo, Hideaki; Mizunoya, Wataru; Ikeuchi, Yoshihide; Tatsumi, Ryuichi.
In: Animal science journal = Nihon chikusan Gakkaihō, Vol. 85, No. 12, 01.12.2014, p. 994-1000.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration
AU - Sawano, Shoko
AU - Suzuki, Takahiro
AU - Do, Mai Khoi Q.
AU - Ohtsubo, Hideaki
AU - Mizunoya, Wataru
AU - Ikeuchi, Yoshihide
AU - Tatsumi, Ryuichi
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Regenerative intramuscular motor-innervation is thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies showed that resident myogenic stem cells, satellite cells, up-regulate a secreted neural-chemorepellent semaphorin 3A (Sema3A) during the early-differentiation period, in response to hepatocyte growth factor (HGF) elevated in injured muscle. However, a paracrine source of the HGF release is still unknown. Very recently, we proposed a possible contribution of anti-inflammatory macrophages (CD206-positive M2) by showing that M2 cells infiltrate predominantly at the early-differentiation phase (3-5 days post-injury) and produce/secrete large amounts of HGF. However, in understanding this concept there still remains a critical need to examine if phagocytotic pro-inflammatory macrophages (CD86-positive M1), another activated-phenotype still present at the early-differentiation phase concerned, produce HGF upon muscle injury. The current immunocytochemical study demonstrated that the HGF expression is negative for M1 prepared from cardiotoxin-injured Tibialis anterior muscle at day 5, in contrast to the intense fluorescent-signal of M2 served as a positive control. This supplementary result advances our understanding of a spatiotemporal burst of HGF secretion from M2 populations (not M1) to impact Sema3A expression, which ensures a coordinated delay in attachment of motoneuron terminals onto damaged and generating fibers during the early phase of muscle regeneration.
AB - Regenerative intramuscular motor-innervation is thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies showed that resident myogenic stem cells, satellite cells, up-regulate a secreted neural-chemorepellent semaphorin 3A (Sema3A) during the early-differentiation period, in response to hepatocyte growth factor (HGF) elevated in injured muscle. However, a paracrine source of the HGF release is still unknown. Very recently, we proposed a possible contribution of anti-inflammatory macrophages (CD206-positive M2) by showing that M2 cells infiltrate predominantly at the early-differentiation phase (3-5 days post-injury) and produce/secrete large amounts of HGF. However, in understanding this concept there still remains a critical need to examine if phagocytotic pro-inflammatory macrophages (CD86-positive M1), another activated-phenotype still present at the early-differentiation phase concerned, produce HGF upon muscle injury. The current immunocytochemical study demonstrated that the HGF expression is negative for M1 prepared from cardiotoxin-injured Tibialis anterior muscle at day 5, in contrast to the intense fluorescent-signal of M2 served as a positive control. This supplementary result advances our understanding of a spatiotemporal burst of HGF secretion from M2 populations (not M1) to impact Sema3A expression, which ensures a coordinated delay in attachment of motoneuron terminals onto damaged and generating fibers during the early phase of muscle regeneration.
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U2 - 10.1111/asj.12264
DO - 10.1111/asj.12264
M3 - Article
C2 - 25185534
AN - SCOPUS:85027918581
VL - 85
SP - 994
EP - 1000
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 12
ER -