Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene

H. Kato, Y. Zhou, Kazuo Asanoma, H. Kondo, Y. Yoshikawa, K. Watanabe, T. Matsuda, N. Wake, J. C. Barrett

Research output: Contribution to journalArticle

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Abstract

To obtain functional evidence for DCC as a tumour suppressor associated with endometrial cancer, the human DCC cDNA encoding a complete open reading frame (ORF) was transfected into highly tumorigenic human endometrial carcinoma cells, HHUA and Ishikawa in which DCC expression was completely deleted. Reconstituted expression of DCC in HHUA had little effect on in vitro growth, but suppressed tumour formation in mice completely. The clones from Ishikawa had abundant DCC expression similar to that in normal endometrium. Their growth in vitro was suppressed and showed apoptotic phenotype. Lower levels of DCC expression in the prolonged passaged clones did not induce apoptosis, but still had the potential to suppress tumorigenicity. These observations imply a role of DCC in regulation of normal endometrial cell growth, and categorize DCC as the tumour suppressor gene for endometrial cancer.

Original languageEnglish
Pages (from-to)459-466
Number of pages8
JournalBritish journal of cancer
Volume82
Issue number2
DOIs
Publication statusPublished - Jan 1 2000

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DCC Genes
Endometrial Neoplasms
Growth
Clone Cells
Endometrium
Tumor Suppressor Genes
Open Reading Frames
Neoplasms
Complementary DNA
Apoptosis
Phenotype
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene. / Kato, H.; Zhou, Y.; Asanoma, Kazuo; Kondo, H.; Yoshikawa, Y.; Watanabe, K.; Matsuda, T.; Wake, N.; Barrett, J. C.

In: British journal of cancer, Vol. 82, No. 2, 01.01.2000, p. 459-466.

Research output: Contribution to journalArticle

Kato, H, Zhou, Y, Asanoma, K, Kondo, H, Yoshikawa, Y, Watanabe, K, Matsuda, T, Wake, N & Barrett, JC 2000, 'Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene', British journal of cancer, vol. 82, no. 2, pp. 459-466. https://doi.org/10.1054/bjoc.1999.0943
Kato, H. ; Zhou, Y. ; Asanoma, Kazuo ; Kondo, H. ; Yoshikawa, Y. ; Watanabe, K. ; Matsuda, T. ; Wake, N. ; Barrett, J. C. / Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene. In: British journal of cancer. 2000 ; Vol. 82, No. 2. pp. 459-466.
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