Suppression of DNA replication via mos function during meiotic divisions in xenopus oocytes

Nobuaki Furuno, Mayumi Nishizawa, Kenji Okazaki, Hirotaka Tanaka, Jun Iwashita, Nobushige Nakajo, Yasuki Ogawa, Noriyuki Sagata

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202 Citations (Scopus)

Abstract

Meiosis is characterized by the absence of DNA replication between the two successive divisions. In Xenopus eggs, the ability to replicate DNA develops during meiotic maturation, but is normally suppressed until fertilization. Here we show that development of the DNA-replicating ability depends on new protein synthesis during meiosis I, and that mere ablation of the endogenous c-mos product Mos allows maturing oocytes to enter interphase and replicate DNA just after meiosis I. Moreover, we demonstrate that during normal maturation cdc-2 kinase undergoes precocious inactivation in meiosis I and then premature reactivation before meiosis II; importantly, this premature cdc-2 reactivation absolutely requires Mos function and its direct inhibition by a dominant-negative cdc-2 mutant also results in nuclear reformation and DNA replication immediately after meiosis I. These findings indicate that suppression of DNA replication during meiotic divisions in Xenopus oocytes is accomplished by the Mos-mediated premature reactivation of cdc-2 kinase. We suggest that these mechanisms for suppressing DNA replication may be specific for meiosis in animal oocytes, and that the ultimate biological function, including the well known cytostatic factor activity, of Mos during meiotic maturation may be to prevent undesirable DNA replication or parthenogenetic activation before fertilization.

Original languageEnglish
Pages (from-to)2399-2410
Number of pages12
JournalEMBO Journal
Volume13
Issue number10
DOIs
Publication statusPublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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    Furuno, N., Nishizawa, M., Okazaki, K., Tanaka, H., Iwashita, J., Nakajo, N., Ogawa, Y., & Sagata, N. (1994). Suppression of DNA replication via mos function during meiotic divisions in xenopus oocytes. EMBO Journal, 13(10), 2399-2410. https://doi.org/10.1002/j.1460-2075.1994.tb06524.x