Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin

Makiko Yamashita, Gakuro Harada, Shin ei Matsumoto, Yoshihiro Aiba, Akira Ichikawa, Tsukasa Fujiki, Miyako Udono, Shigeru Kabayama, Tadashi Yoshida, Pingbo Zhang, Hiroshi Fujii, Sanetaka Shirahata, Yoshinori Katakura

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3 Citations (Scopus)

Abstract

In vitro antigen stimulation of peripheral blood mononuclear cells (PBMCs) does not induce immunoglobulin (Ig) production. However, pretreatment of PBMCs with l-leucyl-l-leucine methyl ester (LLME) prior to in vitro stimulation removes the suppression of Ig production. In the present study, we attempted to identify the target cells of LLME and determine the mechanisms by which Ig production in PBMCs is suppressed. We found that CD14+ monocytes are involved in the suppression of Ig production in PBMCs. Furthermore, we confirmed that heavy-chain ferritin derived from CD14+ monocytes suppresses Ig production in PBMCs, possibly through iron sequestration.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalImmunobiology
Volume219
Issue number2
DOIs
Publication statusPublished - Feb 1 2014

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Hematology

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