TY - JOUR
T1 - Suppression of invasion and metastasis in aggressive salivary cancer cells through targeted inhibition of ID1 gene expression
AU - Murase, Ryuichi
AU - Sumida, Tomoki
AU - Kawamura, Rumi
AU - Onishi-Ishikawa, Akiko
AU - Hamakawa, Hiroyuki
AU - McAllister, Sean D.
AU - Desprez, Pierre Yves
N1 - Funding Information:
This work was supported by the Japanese Government Overseas Study Program and Grant-in-Aid for Scientific Research from The Japanese Ministry of Education, Culture, Sports, Science and Technology ( 15K11257 ) (to TS), and by the National Institutes of Health ( CA082548 , CA135281 ) and the Komen Breast Cancer Research Foundation ( KG090385 ) (to SDM and PYD).
Publisher Copyright:
© 2016 Elsevier Ireland Ltd.
PY - 2016/7/10
Y1 - 2016/7/10
N2 - Salivary gland cancer (SGC) represents the most common malignancy in the head and neck region, and often metastasizes to the lungs. The helix-loop-helix ID1 protein has been shown to control metastatic progression in many types of cancers. Using two different approaches to target the expression of ID1 (genetic knockdown and progesterone receptor introduction combined with progesterone treatment), we previously determined that the aggressiveness of salivary gland tumor ACCM cells in culture was suppressed. Here, using the same approaches to target ID1 expression, we investigated the ability of ACCM cells to generate lung metastatic foci in nude mice. Moreover, since both approaches would be challenging for applications in humans, we added a third approach, i.e., treatment of mice with a non-toxic cannabinoid compound known to down-regulate ID1 gene expression. All approaches aimed at targeting the pro-metastatic ID1 gene led to a significant reduction in the formation of lung metastatic foci. Therefore, targeting a key transcriptional regulator using different means results in the same reduction of the metastatic spread of SGC cells in animal models, suggesting a novel approach for the treatment of patients with aggressive SGC.
AB - Salivary gland cancer (SGC) represents the most common malignancy in the head and neck region, and often metastasizes to the lungs. The helix-loop-helix ID1 protein has been shown to control metastatic progression in many types of cancers. Using two different approaches to target the expression of ID1 (genetic knockdown and progesterone receptor introduction combined with progesterone treatment), we previously determined that the aggressiveness of salivary gland tumor ACCM cells in culture was suppressed. Here, using the same approaches to target ID1 expression, we investigated the ability of ACCM cells to generate lung metastatic foci in nude mice. Moreover, since both approaches would be challenging for applications in humans, we added a third approach, i.e., treatment of mice with a non-toxic cannabinoid compound known to down-regulate ID1 gene expression. All approaches aimed at targeting the pro-metastatic ID1 gene led to a significant reduction in the formation of lung metastatic foci. Therefore, targeting a key transcriptional regulator using different means results in the same reduction of the metastatic spread of SGC cells in animal models, suggesting a novel approach for the treatment of patients with aggressive SGC.
UR - http://www.scopus.com/inward/record.url?scp=84963803947&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963803947&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2016.04.021
DO - 10.1016/j.canlet.2016.04.021
M3 - Article
C2 - 27087608
AN - SCOPUS:84963803947
VL - 377
SP - 11
EP - 16
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -