Suppression of oxidative stress after transient focal ischemia in interleukin-1 knock out mice

H. Ohtaki, Atsushi Takaki, L. Yin, K. Dohi, T. Nakamachi, M. Matsunaga, R. Horai, M. Asano, Y. Iwakura, S. Shioda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Interleukin-l (IL-1) contributes to ischemic neurodegeneration. However, the mechanisms regulating action of IL-1 are still poorly understood. In order to clarify this central issue, mice that were gene deficient both IL-1α and β (IL-1 KO) and wild-type mice were subjected to 1 hour transient middle cerebral artery occlusion (tMCAO). The concentration of 8-hydroxy deoxyguanosine (80HdG) which is considered to be a reliable oxidative DNA damage by superoxide anion, in brain and of total nitric oxide (NO) in plasma were determined by use of HPLC. Twenty-four hours after tMCAO, the ratio of 80HdG to dG in the ipsilateral hemisphere of wild-type mice were 2.24 × 10 -3 and 4.41 × 10-3 in the neocortex and striatum, respectively. The concentration of 80HdG in the ipsilateral hemisphere of the wild-type mice was higher than that of the IL-1 KO mice. The concentration of total NO in the plasma of IL-1 KO mice was also lower than that of the wild-type 24 hours after tMCAO. These results strongly suggest that IL-1 is participated in generating reactive oxygen spices and it aggravates and induces the ischemic neuronal cell death. (183 words)

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalActa Neurochirurgica, Supplementum
Issue number86
DOIs
Publication statusPublished - Jan 1 2003

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Interleukin-1
Knockout Mice
Oxidative Stress
Ischemia
Middle Cerebral Artery Infarction
Nitric Oxide
Spices
Deoxyguanosine
Interleukins
Neocortex
Superoxides
DNA Damage
Cell Death
High Pressure Liquid Chromatography
Oxygen
Brain
Genes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

Cite this

Suppression of oxidative stress after transient focal ischemia in interleukin-1 knock out mice. / Ohtaki, H.; Takaki, Atsushi; Yin, L.; Dohi, K.; Nakamachi, T.; Matsunaga, M.; Horai, R.; Asano, M.; Iwakura, Y.; Shioda, S.

In: Acta Neurochirurgica, Supplementum, No. 86, 01.01.2003, p. 191-194.

Research output: Contribution to journalArticle

Ohtaki, H, Takaki, A, Yin, L, Dohi, K, Nakamachi, T, Matsunaga, M, Horai, R, Asano, M, Iwakura, Y & Shioda, S 2003, 'Suppression of oxidative stress after transient focal ischemia in interleukin-1 knock out mice', Acta Neurochirurgica, Supplementum, no. 86, pp. 191-194. https://doi.org/10.1007/978-3-7091-0651-8_41
Ohtaki, H. ; Takaki, Atsushi ; Yin, L. ; Dohi, K. ; Nakamachi, T. ; Matsunaga, M. ; Horai, R. ; Asano, M. ; Iwakura, Y. ; Shioda, S. / Suppression of oxidative stress after transient focal ischemia in interleukin-1 knock out mice. In: Acta Neurochirurgica, Supplementum. 2003 ; No. 86. pp. 191-194.
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