Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

Yupin Li, Takeki Hamasaki, Noboru Nakamichi, Taichi Kashiwagi, Takaaki Komatsu, Jun Ye, Kiichiro Teruya, Masumi Abe, Hanxu Yan, Tomoya Kinjo, Shigeru Kabayama, Munenori Kawamura, Sanetaka Shirahata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.

Original languageEnglish
Pages (from-to)119-131
Number of pages13
JournalCytotechnology
Volume63
Issue number2
DOIs
Publication statusPublished - Mar 1 2011

Fingerprint

Alloxan
Cell death
Medical problems
Type 1 Diabetes Mellitus
Apoptosis
Reactive Oxygen Species
Water
Oxygen
Insulin
Scavenging
G1 Phase
DNA Fragmentation
Glucose
Blood Glucose
Diabetes Mellitus
Animals
DNA
Blood

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Li, Y., Hamasaki, T., Nakamichi, N., Kashiwagi, T., Komatsu, T., Ye, J., ... Shirahata, S. (2011). Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus. Cytotechnology, 63(2), 119-131. https://doi.org/10.1007/s10616-010-9317-6

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus. / Li, Yupin; Hamasaki, Takeki; Nakamichi, Noboru; Kashiwagi, Taichi; Komatsu, Takaaki; Ye, Jun; Teruya, Kiichiro; Abe, Masumi; Yan, Hanxu; Kinjo, Tomoya; Kabayama, Shigeru; Kawamura, Munenori; Shirahata, Sanetaka.

In: Cytotechnology, Vol. 63, No. 2, 01.03.2011, p. 119-131.

Research output: Contribution to journalArticle

Li, Y, Hamasaki, T, Nakamichi, N, Kashiwagi, T, Komatsu, T, Ye, J, Teruya, K, Abe, M, Yan, H, Kinjo, T, Kabayama, S, Kawamura, M & Shirahata, S 2011, 'Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus', Cytotechnology, vol. 63, no. 2, pp. 119-131. https://doi.org/10.1007/s10616-010-9317-6
Li, Yupin ; Hamasaki, Takeki ; Nakamichi, Noboru ; Kashiwagi, Taichi ; Komatsu, Takaaki ; Ye, Jun ; Teruya, Kiichiro ; Abe, Masumi ; Yan, Hanxu ; Kinjo, Tomoya ; Kabayama, Shigeru ; Kawamura, Munenori ; Shirahata, Sanetaka. / Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus. In: Cytotechnology. 2011 ; Vol. 63, No. 2. pp. 119-131.
@article{0c46301d7e324211b6ee24a2eead861d,
title = "Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus",
abstract = "Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.",
author = "Yupin Li and Takeki Hamasaki and Noboru Nakamichi and Taichi Kashiwagi and Takaaki Komatsu and Jun Ye and Kiichiro Teruya and Masumi Abe and Hanxu Yan and Tomoya Kinjo and Shigeru Kabayama and Munenori Kawamura and Sanetaka Shirahata",
year = "2011",
month = "3",
day = "1",
doi = "10.1007/s10616-010-9317-6",
language = "English",
volume = "63",
pages = "119--131",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

AU - Li, Yupin

AU - Hamasaki, Takeki

AU - Nakamichi, Noboru

AU - Kashiwagi, Taichi

AU - Komatsu, Takaaki

AU - Ye, Jun

AU - Teruya, Kiichiro

AU - Abe, Masumi

AU - Yan, Hanxu

AU - Kinjo, Tomoya

AU - Kabayama, Shigeru

AU - Kawamura, Munenori

AU - Shirahata, Sanetaka

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.

AB - Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.

UR - http://www.scopus.com/inward/record.url?scp=79955903247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955903247&partnerID=8YFLogxK

U2 - 10.1007/s10616-010-9317-6

DO - 10.1007/s10616-010-9317-6

M3 - Article

C2 - 21063772

AN - SCOPUS:79955903247

VL - 63

SP - 119

EP - 131

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 2

ER -