Suppressive effects of natural reduced waters on alloxan-induced apoptosis and type 1 diabetes mellitus

Yuping Li, Takeki Hamasaki, Kiichiro Teruya, Noboru Nakamichi, Zbigniew Gadek, Taichi Kashiwagi, Hanxu Yan, Tomoya Kinjo, Takaaki Komatsu, Yoshitoki Ishii, Sanetaka Shirahata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Insulin-producing cells express limited activities of anti-oxidative enzymes. Therefore, reactive oxygen species (ROS) produced in these cells play a crucial role in cytotoxic effects. Furthermore, diabetes mellitus (DM) development is closely linked to higher ROS levels in insulin-producing cells. Hita Tenryosui Water® (Hita T. W., Hita, Japan) and Nordenau water (Nord. W., Nordenau, Germany), referred to as natural reduced waters (NRWs), scavenge ROS in cultured cells, and therefore, might be a possibility as an alternative to conventional pharmacological agents against DM. Therefore, this study aimed to investigate the role of NRWs in alloxan (ALX)-induced β-cell apoptosis as well as in ALX-induced diabetic mice. NRWs equally suppressed DNA fragmentation levels. Hita T. W. and Nord. W. ameliorated ALX-induced sub-G1 phase production from approximately 40% of control levels to 8.5 and 11.8%, respectively. NRWs restored serum insulin levels (p<0.01) and reduced blood glucose levels (p<0.01) in ALX-induced mice. Hita T. W. restored tissue superoxide dismutase (SOD) (p<0.05) activity but not tissue catalase activity. Hita T. W. did not elevate SOD or catalase activity in HIT-T15 cells. Nord. W. restored SOD (p<0.05) and catalase (p<0.05) activity in both cultured cells and pancreatic tissue to normal levels. Even though variable efficacies were observed between Hita T. W. and Nord. W., both waters suppressed ALX-induced DM development in CD-1 male mice by administering NRWs for 8 weeks. Our results suggest that Hita T. W. and Nord. W. protect against ALX-induced β-cell apoptosis, and prevent the development of ALX-induced DM in experimental animals by regulating ALX-derived ROS generation and elevating antioxidative enzymes. Therefore, the two NRWs tested here are promising candidates for the prevention of DM development.

Original languageEnglish
Pages (from-to)281-297
Number of pages17
JournalCytotechnology
Volume64
Issue number3
DOIs
Publication statusPublished - May 2012

Fingerprint

Alloxan
Cell death
Medical problems
Type 1 Diabetes Mellitus
Apoptosis
Water
Diabetes Mellitus
Reactive Oxygen Species
Insulin
Catalase
Superoxide Dismutase
Oxygen
Experimental Diabetes Mellitus
Tissue
Cultured Cells
Enzymes
Cells
Level control
G1 Phase
DNA Fragmentation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Suppressive effects of natural reduced waters on alloxan-induced apoptosis and type 1 diabetes mellitus. / Li, Yuping; Hamasaki, Takeki; Teruya, Kiichiro; Nakamichi, Noboru; Gadek, Zbigniew; Kashiwagi, Taichi; Yan, Hanxu; Kinjo, Tomoya; Komatsu, Takaaki; Ishii, Yoshitoki; Shirahata, Sanetaka.

In: Cytotechnology, Vol. 64, No. 3, 05.2012, p. 281-297.

Research output: Contribution to journalArticle

Li, Y, Hamasaki, T, Teruya, K, Nakamichi, N, Gadek, Z, Kashiwagi, T, Yan, H, Kinjo, T, Komatsu, T, Ishii, Y & Shirahata, S 2012, 'Suppressive effects of natural reduced waters on alloxan-induced apoptosis and type 1 diabetes mellitus', Cytotechnology, vol. 64, no. 3, pp. 281-297. https://doi.org/10.1007/s10616-011-9414-1
Li, Yuping ; Hamasaki, Takeki ; Teruya, Kiichiro ; Nakamichi, Noboru ; Gadek, Zbigniew ; Kashiwagi, Taichi ; Yan, Hanxu ; Kinjo, Tomoya ; Komatsu, Takaaki ; Ishii, Yoshitoki ; Shirahata, Sanetaka. / Suppressive effects of natural reduced waters on alloxan-induced apoptosis and type 1 diabetes mellitus. In: Cytotechnology. 2012 ; Vol. 64, No. 3. pp. 281-297.
@article{5baddd0a888b46c7bdfa38a105ca47b5,
title = "Suppressive effects of natural reduced waters on alloxan-induced apoptosis and type 1 diabetes mellitus",
abstract = "Insulin-producing cells express limited activities of anti-oxidative enzymes. Therefore, reactive oxygen species (ROS) produced in these cells play a crucial role in cytotoxic effects. Furthermore, diabetes mellitus (DM) development is closely linked to higher ROS levels in insulin-producing cells. Hita Tenryosui Water{\circledR} (Hita T. W., Hita, Japan) and Nordenau water (Nord. W., Nordenau, Germany), referred to as natural reduced waters (NRWs), scavenge ROS in cultured cells, and therefore, might be a possibility as an alternative to conventional pharmacological agents against DM. Therefore, this study aimed to investigate the role of NRWs in alloxan (ALX)-induced β-cell apoptosis as well as in ALX-induced diabetic mice. NRWs equally suppressed DNA fragmentation levels. Hita T. W. and Nord. W. ameliorated ALX-induced sub-G1 phase production from approximately 40{\%} of control levels to 8.5 and 11.8{\%}, respectively. NRWs restored serum insulin levels (p<0.01) and reduced blood glucose levels (p<0.01) in ALX-induced mice. Hita T. W. restored tissue superoxide dismutase (SOD) (p<0.05) activity but not tissue catalase activity. Hita T. W. did not elevate SOD or catalase activity in HIT-T15 cells. Nord. W. restored SOD (p<0.05) and catalase (p<0.05) activity in both cultured cells and pancreatic tissue to normal levels. Even though variable efficacies were observed between Hita T. W. and Nord. W., both waters suppressed ALX-induced DM development in CD-1 male mice by administering NRWs for 8 weeks. Our results suggest that Hita T. W. and Nord. W. protect against ALX-induced β-cell apoptosis, and prevent the development of ALX-induced DM in experimental animals by regulating ALX-derived ROS generation and elevating antioxidative enzymes. Therefore, the two NRWs tested here are promising candidates for the prevention of DM development.",
author = "Yuping Li and Takeki Hamasaki and Kiichiro Teruya and Noboru Nakamichi and Zbigniew Gadek and Taichi Kashiwagi and Hanxu Yan and Tomoya Kinjo and Takaaki Komatsu and Yoshitoki Ishii and Sanetaka Shirahata",
year = "2012",
month = "5",
doi = "10.1007/s10616-011-9414-1",
language = "English",
volume = "64",
pages = "281--297",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Suppressive effects of natural reduced waters on alloxan-induced apoptosis and type 1 diabetes mellitus

AU - Li, Yuping

AU - Hamasaki, Takeki

AU - Teruya, Kiichiro

AU - Nakamichi, Noboru

AU - Gadek, Zbigniew

AU - Kashiwagi, Taichi

AU - Yan, Hanxu

AU - Kinjo, Tomoya

AU - Komatsu, Takaaki

AU - Ishii, Yoshitoki

AU - Shirahata, Sanetaka

PY - 2012/5

Y1 - 2012/5

N2 - Insulin-producing cells express limited activities of anti-oxidative enzymes. Therefore, reactive oxygen species (ROS) produced in these cells play a crucial role in cytotoxic effects. Furthermore, diabetes mellitus (DM) development is closely linked to higher ROS levels in insulin-producing cells. Hita Tenryosui Water® (Hita T. W., Hita, Japan) and Nordenau water (Nord. W., Nordenau, Germany), referred to as natural reduced waters (NRWs), scavenge ROS in cultured cells, and therefore, might be a possibility as an alternative to conventional pharmacological agents against DM. Therefore, this study aimed to investigate the role of NRWs in alloxan (ALX)-induced β-cell apoptosis as well as in ALX-induced diabetic mice. NRWs equally suppressed DNA fragmentation levels. Hita T. W. and Nord. W. ameliorated ALX-induced sub-G1 phase production from approximately 40% of control levels to 8.5 and 11.8%, respectively. NRWs restored serum insulin levels (p<0.01) and reduced blood glucose levels (p<0.01) in ALX-induced mice. Hita T. W. restored tissue superoxide dismutase (SOD) (p<0.05) activity but not tissue catalase activity. Hita T. W. did not elevate SOD or catalase activity in HIT-T15 cells. Nord. W. restored SOD (p<0.05) and catalase (p<0.05) activity in both cultured cells and pancreatic tissue to normal levels. Even though variable efficacies were observed between Hita T. W. and Nord. W., both waters suppressed ALX-induced DM development in CD-1 male mice by administering NRWs for 8 weeks. Our results suggest that Hita T. W. and Nord. W. protect against ALX-induced β-cell apoptosis, and prevent the development of ALX-induced DM in experimental animals by regulating ALX-derived ROS generation and elevating antioxidative enzymes. Therefore, the two NRWs tested here are promising candidates for the prevention of DM development.

AB - Insulin-producing cells express limited activities of anti-oxidative enzymes. Therefore, reactive oxygen species (ROS) produced in these cells play a crucial role in cytotoxic effects. Furthermore, diabetes mellitus (DM) development is closely linked to higher ROS levels in insulin-producing cells. Hita Tenryosui Water® (Hita T. W., Hita, Japan) and Nordenau water (Nord. W., Nordenau, Germany), referred to as natural reduced waters (NRWs), scavenge ROS in cultured cells, and therefore, might be a possibility as an alternative to conventional pharmacological agents against DM. Therefore, this study aimed to investigate the role of NRWs in alloxan (ALX)-induced β-cell apoptosis as well as in ALX-induced diabetic mice. NRWs equally suppressed DNA fragmentation levels. Hita T. W. and Nord. W. ameliorated ALX-induced sub-G1 phase production from approximately 40% of control levels to 8.5 and 11.8%, respectively. NRWs restored serum insulin levels (p<0.01) and reduced blood glucose levels (p<0.01) in ALX-induced mice. Hita T. W. restored tissue superoxide dismutase (SOD) (p<0.05) activity but not tissue catalase activity. Hita T. W. did not elevate SOD or catalase activity in HIT-T15 cells. Nord. W. restored SOD (p<0.05) and catalase (p<0.05) activity in both cultured cells and pancreatic tissue to normal levels. Even though variable efficacies were observed between Hita T. W. and Nord. W., both waters suppressed ALX-induced DM development in CD-1 male mice by administering NRWs for 8 weeks. Our results suggest that Hita T. W. and Nord. W. protect against ALX-induced β-cell apoptosis, and prevent the development of ALX-induced DM in experimental animals by regulating ALX-derived ROS generation and elevating antioxidative enzymes. Therefore, the two NRWs tested here are promising candidates for the prevention of DM development.

UR - http://www.scopus.com/inward/record.url?scp=84863727478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863727478&partnerID=8YFLogxK

U2 - 10.1007/s10616-011-9414-1

DO - 10.1007/s10616-011-9414-1

M3 - Article

C2 - 22143345

AN - SCOPUS:84863727478

VL - 64

SP - 281

EP - 297

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 3

ER -