Surf4 modulates STIM1-dependent calcium entry

Yoko Fujii, Masayuki Shiota, Yasuyuki Ohkawa, Akemi Baba, Hideki Wanibuchi, Tatsuo Kinashi, Tomohiro Kurosaki, Yoshihiro Baba

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Store-operated Ca 2+ entry (SOCE) is crucial for various physiological responses in immune cells. Although it is known that STIM1 relocates into discrete puncta juxtaposed to the plasma membrane to initiate SOCE, the machinery modulating the function of STIM1 remains unclear. We explored to find its modulators using affinity purification for STIM1-binding proteins and identified surfeit locus protein 4 (Surf4). Surf4 associated with STIM1 in the endoplasmic reticulum. Deletion of Surf4 in DT40 B cells resulted in marked increase of SOCE and facilitation of STIM1 clustering upon store-depletion. These findings suggest the modulatory function of Surf4 for STIM1-mediated SOCE.

Original languageEnglish
Pages (from-to)615-620
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume422
Issue number4
DOIs
Publication statusPublished - Jun 15 2012

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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