Surface adhesion of salmonella enterica and staphylococcus aureus under fluctuating temperatures

D. Hamanaka, K. Morimatsu, Tanaka Fumihiko, Toshitaka Uchino

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The bacterial adherent characteristics of two pathogens (Salmonella enterica, Staphylococcus aureus) on the surface of polystyrene were investigated under a fluctuating temperature regime and different nutrient levels (trypticase soy broth). Bacterial adhesions were evaluated by the optical density value of bacterial cells stained with 0.1% (w/v) of crystal violet solution. Under constant temperature conditions at 5 and 30°C, the adherent potentials of both S. enterica and S. aureus were stable at the end of incubation (5 days). Under a fluctuating temperature regime, the cell adhesion potentials were changed with the period of incubation. The adhesions of both pathogens were considerably increased by rapid temperature elevation from 5 to 30°C, while approximately twice the optical density value of cell adhesion of S. enterica was observed on the polystyrene surface compared with constant conditions. Transferring the sample tube to 5°C after fluctuation from 5 to 30°C led to considerable cell detachment of S. enterica by the end of incubation. It was probable that S. enterica cells used in this study was extremely sensitive to environmental variation, consequently cell adhesion and release could be observed under fluctuating temperature conditions. Meanwhile, stable adhesion of S. aureus cells was observed at 5°C after temperature fluctuations from 5 to 30°C.

Original languageEnglish
Title of host publicationInternational Symposium Postharvest Pacifica 2009 - Pathways to Quality
Subtitle of host publicationV International Symposium on Managing Quality in Chains + Australasian Postharvest Horticultural Conference
PublisherInternational Society for Horticultural Science
Pages541-546
Number of pages6
ISBN (Print)9789066056039
DOIs
Publication statusPublished - Nov 25 2010

Publication series

NameActa Horticulturae
Volume880
ISSN (Print)0567-7572

Fingerprint

Salmonella enterica
adhesion
Staphylococcus aureus
cell adhesion
temperature
polystyrenes
absorbance
cells
bacterial adhesion
gentian violet
pathogens
nutrients

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Hamanaka, D., Morimatsu, K., Fumihiko, T., & Uchino, T. (2010). Surface adhesion of salmonella enterica and staphylococcus aureus under fluctuating temperatures. In International Symposium Postharvest Pacifica 2009 - Pathways to Quality: V International Symposium on Managing Quality in Chains + Australasian Postharvest Horticultural Conference (pp. 541-546). (Acta Horticulturae; Vol. 880). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2010.880.65

Surface adhesion of salmonella enterica and staphylococcus aureus under fluctuating temperatures. / Hamanaka, D.; Morimatsu, K.; Fumihiko, Tanaka; Uchino, Toshitaka.

International Symposium Postharvest Pacifica 2009 - Pathways to Quality: V International Symposium on Managing Quality in Chains + Australasian Postharvest Horticultural Conference. International Society for Horticultural Science, 2010. p. 541-546 (Acta Horticulturae; Vol. 880).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hamanaka, D, Morimatsu, K, Fumihiko, T & Uchino, T 2010, Surface adhesion of salmonella enterica and staphylococcus aureus under fluctuating temperatures. in International Symposium Postharvest Pacifica 2009 - Pathways to Quality: V International Symposium on Managing Quality in Chains + Australasian Postharvest Horticultural Conference. Acta Horticulturae, vol. 880, International Society for Horticultural Science, pp. 541-546. https://doi.org/10.17660/ActaHortic.2010.880.65
Hamanaka D, Morimatsu K, Fumihiko T, Uchino T. Surface adhesion of salmonella enterica and staphylococcus aureus under fluctuating temperatures. In International Symposium Postharvest Pacifica 2009 - Pathways to Quality: V International Symposium on Managing Quality in Chains + Australasian Postharvest Horticultural Conference. International Society for Horticultural Science. 2010. p. 541-546. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2010.880.65
Hamanaka, D. ; Morimatsu, K. ; Fumihiko, Tanaka ; Uchino, Toshitaka. / Surface adhesion of salmonella enterica and staphylococcus aureus under fluctuating temperatures. International Symposium Postharvest Pacifica 2009 - Pathways to Quality: V International Symposium on Managing Quality in Chains + Australasian Postharvest Horticultural Conference. International Society for Horticultural Science, 2010. pp. 541-546 (Acta Horticulturae).
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