Surface force and vibrational spectroscopic analyses of interfacial water molecules in the vicinity of methoxy-tri(ethylene glycol)-terminated monolayers: mechanisms underlying the effect of lateral packing density on bioinertness

Taito Sekine, Syifa Asatyas, Chikako Sato, Shigeaki Morita, Masaru Tanaka, Tomohiro Hayashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Unequivocal dependence of bioinertness of self-assembled monolayers of methoxy-tri(ethylene glycol)-terminated alkanethiol (EG3-OMe SAMs) on their packing density has been a mystery for more than two decades. We tackled this long-standing question by performing surface force and surface-enhanced infrared absorption (SEIRA) spectroscopic measurements. Our surface force measurements revealed a physical barrier of interfacial water in the vicinity of the Au-supported EG3-OMe SAM (low packing density), whereas the Ag-supported one (high packing density) did not possess such interfacial water. In addition, the results of SEIRA measurements clearly exhibited that hydrogen bonding states of the interfacial water differ depending on the substrates. We also characterized the bioinertness of these SAMs by protein adsorption tests and adhesion assays of platelet and human umbilical vein endothelial cells. The hydrogen bonding states of the interfacial water and water-induced interaction clearly correlated with the bioinertness of the SAMs, suggesting that the interfacial water plays an important role determining the interaction of the SAMs with biomolecules and cells.

Original languageEnglish
Pages (from-to)1231-1243
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Volume28
Issue number10-12
DOIs
Publication statusPublished - Aug 13 2017

Fingerprint

Ethylene Glycol
Ethylene glycol
Monolayers
Molecules
Water
Infrared absorption
Hydrogen Bonding
Hydrogen bonds
Architectural Accessibility
Force measurement
Surface measurement
Endothelial cells
Human Umbilical Vein Endothelial Cells
Biomolecules
Self assembled monolayers
Platelets
Adsorption
Assays
Blood Platelets
Adhesion

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

@article{f109de7b28524a55ae78f14173193033,
title = "Surface force and vibrational spectroscopic analyses of interfacial water molecules in the vicinity of methoxy-tri(ethylene glycol)-terminated monolayers: mechanisms underlying the effect of lateral packing density on bioinertness",
abstract = "Unequivocal dependence of bioinertness of self-assembled monolayers of methoxy-tri(ethylene glycol)-terminated alkanethiol (EG3-OMe SAMs) on their packing density has been a mystery for more than two decades. We tackled this long-standing question by performing surface force and surface-enhanced infrared absorption (SEIRA) spectroscopic measurements. Our surface force measurements revealed a physical barrier of interfacial water in the vicinity of the Au-supported EG3-OMe SAM (low packing density), whereas the Ag-supported one (high packing density) did not possess such interfacial water. In addition, the results of SEIRA measurements clearly exhibited that hydrogen bonding states of the interfacial water differ depending on the substrates. We also characterized the bioinertness of these SAMs by protein adsorption tests and adhesion assays of platelet and human umbilical vein endothelial cells. The hydrogen bonding states of the interfacial water and water-induced interaction clearly correlated with the bioinertness of the SAMs, suggesting that the interfacial water plays an important role determining the interaction of the SAMs with biomolecules and cells.",
author = "Taito Sekine and Syifa Asatyas and Chikako Sato and Shigeaki Morita and Masaru Tanaka and Tomohiro Hayashi",
year = "2017",
month = "8",
day = "13",
doi = "10.1080/09205063.2017.1303120",
language = "English",
volume = "28",
pages = "1231--1243",
journal = "Journal of Biomaterials Science, Polymer Edition",
issn = "0920-5063",
publisher = "Taylor and Francis Ltd.",
number = "10-12",

}

TY - JOUR

T1 - Surface force and vibrational spectroscopic analyses of interfacial water molecules in the vicinity of methoxy-tri(ethylene glycol)-terminated monolayers

T2 - mechanisms underlying the effect of lateral packing density on bioinertness

AU - Sekine, Taito

AU - Asatyas, Syifa

AU - Sato, Chikako

AU - Morita, Shigeaki

AU - Tanaka, Masaru

AU - Hayashi, Tomohiro

PY - 2017/8/13

Y1 - 2017/8/13

N2 - Unequivocal dependence of bioinertness of self-assembled monolayers of methoxy-tri(ethylene glycol)-terminated alkanethiol (EG3-OMe SAMs) on their packing density has been a mystery for more than two decades. We tackled this long-standing question by performing surface force and surface-enhanced infrared absorption (SEIRA) spectroscopic measurements. Our surface force measurements revealed a physical barrier of interfacial water in the vicinity of the Au-supported EG3-OMe SAM (low packing density), whereas the Ag-supported one (high packing density) did not possess such interfacial water. In addition, the results of SEIRA measurements clearly exhibited that hydrogen bonding states of the interfacial water differ depending on the substrates. We also characterized the bioinertness of these SAMs by protein adsorption tests and adhesion assays of platelet and human umbilical vein endothelial cells. The hydrogen bonding states of the interfacial water and water-induced interaction clearly correlated with the bioinertness of the SAMs, suggesting that the interfacial water plays an important role determining the interaction of the SAMs with biomolecules and cells.

AB - Unequivocal dependence of bioinertness of self-assembled monolayers of methoxy-tri(ethylene glycol)-terminated alkanethiol (EG3-OMe SAMs) on their packing density has been a mystery for more than two decades. We tackled this long-standing question by performing surface force and surface-enhanced infrared absorption (SEIRA) spectroscopic measurements. Our surface force measurements revealed a physical barrier of interfacial water in the vicinity of the Au-supported EG3-OMe SAM (low packing density), whereas the Ag-supported one (high packing density) did not possess such interfacial water. In addition, the results of SEIRA measurements clearly exhibited that hydrogen bonding states of the interfacial water differ depending on the substrates. We also characterized the bioinertness of these SAMs by protein adsorption tests and adhesion assays of platelet and human umbilical vein endothelial cells. The hydrogen bonding states of the interfacial water and water-induced interaction clearly correlated with the bioinertness of the SAMs, suggesting that the interfacial water plays an important role determining the interaction of the SAMs with biomolecules and cells.

UR - http://www.scopus.com/inward/record.url?scp=85016300883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016300883&partnerID=8YFLogxK

U2 - 10.1080/09205063.2017.1303120

DO - 10.1080/09205063.2017.1303120

M3 - Article

C2 - 28278040

AN - SCOPUS:85016300883

VL - 28

SP - 1231

EP - 1243

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 10-12

ER -