Surface imprinting polymers for the recognition of nucleotides

Hideyuki Tsunemori, Kosuke Araki, Kazuya Uezu, Masahiro Goto, Shintaro Furusaki

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    A highly selective polymer has been prepared for the selective separation of nucleotides by the surface imprinting polymerization. A dialkyl quaternary ammonium chloride was effective as the functional molecule for recognizing the difference in the structure of nucleotides. Adsorptive behavior of the ionic species of the structural analogues, inosine-5'-monophosphoric acid (IMP) and guanosine-5'-monophosphoric acid (GMP), could be controlled by changing the pH condition. Surface imprinting polymers were prepared under different pH conditions; pH 9.0 and pH 8.5. The IMP-imprinted polymers exhibited higher template effect for IMP than for a structural analogue, GMP. A reference polymer prepared without the imprint molecule neither exhibit any selectivity to IMP nor to GMP. The adsorption behavior was quantitatively evaluated by the binding constants for the IMP-imprinted polymer. The imprinting polymer was found to recognize a small structural difference in nucleotides.

    Original languageEnglish
    Pages (from-to)315-321
    Number of pages7
    JournalBioseparation
    Volume10
    Issue number6
    DOIs
    Publication statusPublished - 2001

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Chemical Engineering(all)

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