Surface imprinting polymers for the recognition of nucleotides

Hideyuki Tsunemori, Kosuke Araki, Kazuya Uezu, Masahiro Goto, Shintaro Furusaki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A highly selective polymer has been prepared for the selective separation of nucleotides by the surface imprinting polymerization. A dialkyl quaternary ammonium chloride was effective as the functional molecule for recognizing the difference in the structure of nucleotides. Adsorptive behavior of the ionic species of the structural analogues, inosine-5'-monophosphoric acid (IMP) and guanosine-5'-monophosphoric acid (GMP), could be controlled by changing the pH condition. Surface imprinting polymers were prepared under different pH conditions; pH 9.0 and pH 8.5. The IMP-imprinted polymers exhibited higher template effect for IMP than for a structural analogue, GMP. A reference polymer prepared without the imprint molecule neither exhibit any selectivity to IMP nor to GMP. The adsorption behavior was quantitatively evaluated by the binding constants for the IMP-imprinted polymer. The imprinting polymer was found to recognize a small structural difference in nucleotides.

Original languageEnglish
Pages (from-to)315-321
Number of pages7
JournalBioseparation
Volume10
Issue number6
DOIs
Publication statusPublished - Jan 1 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Chemical Engineering(all)

Fingerprint Dive into the research topics of 'Surface imprinting polymers for the recognition of nucleotides'. Together they form a unique fingerprint.

  • Cite this