Abstract
Glyco-modification of a solid cellulose surface with lactose via an enzymatic reaction was successfully achieved using a surfactant-enveloped enzyme (SEE) in a lithium chloride/dimethylacetamide solvent system. The unique biocatalyst, SEE, which is active in organic media, was prepared by protecting the surface of cellulase with the specific nonionic surfactant dioleyl-N-D-glucona-L-glutamate. Lactose, a second cellulase substrate, was introduced onto cellulose surfaces via SEE-mediated enzymatic reaction. Fluorescent labeling and imaging revealed the presence of galactose residues from lactose on the modified cellulose surface. X-Ray diffractometry showed that the crystal structure of cellulose remained unchanged even after glyco-modification. Cell adhesion assays were carried out using rat liver cells on which galactose-specific receptors are present, and the initial cell attachment (within 12 h) on the lactose-modified cellulose filter was greater than that on an original (unmodified) cellulose matrix. The SEE-mediated biocatalysis enabled efficient glyco-modification of the solid cellulose surface in a one-step reaction, without complicated pre-treatment of the cellulose matrix and donor sugar. Consequently, this simple and effective approach to surface modification of a solid cellulose matrix with bio-functional sugars would be expected to have wide potential applications in glycomaterials engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 1836-1842 |
| Number of pages | 7 |
| Journal | Journal of Materials Chemistry |
| Volume | 19 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - Mar 24 2009 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Materials Chemistry
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Surface modification of a solid-state cellulose matrix with lactose by a surfactant-enveloped enzyme in a nonaqueous medium. / Egusa, Shizuka; Yokota, Shingo; Tanaka, Kyoko; Esaki, Kei; Okutani, Yuri; Ogawa, Yukiko; Kitaoka, Takuya; Goto, Masahiro; Wariishi, Hiroyuki.
In: Journal of Materials Chemistry, Vol. 19, No. 13, 24.03.2009, p. 1836-1842.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surface modification of a solid-state cellulose matrix with lactose by a surfactant-enveloped enzyme in a nonaqueous medium
AU - Egusa, Shizuka
AU - Yokota, Shingo
AU - Tanaka, Kyoko
AU - Esaki, Kei
AU - Okutani, Yuri
AU - Ogawa, Yukiko
AU - Kitaoka, Takuya
AU - Goto, Masahiro
AU - Wariishi, Hiroyuki
PY - 2009/3/24
Y1 - 2009/3/24
N2 - Glyco-modification of a solid cellulose surface with lactose via an enzymatic reaction was successfully achieved using a surfactant-enveloped enzyme (SEE) in a lithium chloride/dimethylacetamide solvent system. The unique biocatalyst, SEE, which is active in organic media, was prepared by protecting the surface of cellulase with the specific nonionic surfactant dioleyl-N-D-glucona-L-glutamate. Lactose, a second cellulase substrate, was introduced onto cellulose surfaces via SEE-mediated enzymatic reaction. Fluorescent labeling and imaging revealed the presence of galactose residues from lactose on the modified cellulose surface. X-Ray diffractometry showed that the crystal structure of cellulose remained unchanged even after glyco-modification. Cell adhesion assays were carried out using rat liver cells on which galactose-specific receptors are present, and the initial cell attachment (within 12 h) on the lactose-modified cellulose filter was greater than that on an original (unmodified) cellulose matrix. The SEE-mediated biocatalysis enabled efficient glyco-modification of the solid cellulose surface in a one-step reaction, without complicated pre-treatment of the cellulose matrix and donor sugar. Consequently, this simple and effective approach to surface modification of a solid cellulose matrix with bio-functional sugars would be expected to have wide potential applications in glycomaterials engineering.
AB - Glyco-modification of a solid cellulose surface with lactose via an enzymatic reaction was successfully achieved using a surfactant-enveloped enzyme (SEE) in a lithium chloride/dimethylacetamide solvent system. The unique biocatalyst, SEE, which is active in organic media, was prepared by protecting the surface of cellulase with the specific nonionic surfactant dioleyl-N-D-glucona-L-glutamate. Lactose, a second cellulase substrate, was introduced onto cellulose surfaces via SEE-mediated enzymatic reaction. Fluorescent labeling and imaging revealed the presence of galactose residues from lactose on the modified cellulose surface. X-Ray diffractometry showed that the crystal structure of cellulose remained unchanged even after glyco-modification. Cell adhesion assays were carried out using rat liver cells on which galactose-specific receptors are present, and the initial cell attachment (within 12 h) on the lactose-modified cellulose filter was greater than that on an original (unmodified) cellulose matrix. The SEE-mediated biocatalysis enabled efficient glyco-modification of the solid cellulose surface in a one-step reaction, without complicated pre-treatment of the cellulose matrix and donor sugar. Consequently, this simple and effective approach to surface modification of a solid cellulose matrix with bio-functional sugars would be expected to have wide potential applications in glycomaterials engineering.
UR - http://www.scopus.com/inward/record.url?scp=62549107079&partnerID=8YFLogxK
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U2 - 10.1039/b819025a
DO - 10.1039/b819025a
M3 - Article
AN - SCOPUS:62549107079
VL - 19
SP - 1836
EP - 1842
JO - Journal of Materials Chemistry
JF - Journal of Materials Chemistry
SN - 0959-9428
IS - 13
ER -