Surface modification of gold nanorods using layer-by-layer technique for cellular uptake

Hironobu Takahashi, Takuro Niidome, Takahito Kawano, Sunao Yamada, Yasuro Niidome

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Gold nanorods (NRs), rod-shaped gold nanoparticles, were modified with bovine serum albumin (BSA) and polyethylenimine (PEI) using layer-by-layer technique. From absorption spectroscopy and zeta potential measurements, it was obvious that NRs were wrapped with these polymers without aggregation of NRs. Following BSA modification, the surface-modified NRs (BSA-NRs) were well-dispersed without aggregation in biochemical conditions, verified from absorption spectroscopy. Further modification with PEI provided positively charged NRs (PEI-NRs). A transmission electron microscopy image of PEI-NRs revealed that the surface modification did not affect changing the shape of the initial NRs. In addition, the PEI-NRs retained the colloidal stability of BSA-NRs in biochemical conditions. We have evaluated that transfection activity of PEI-NRs with HeLa cells. From results of gene expression experiments, it was obvious that the stabilization of NRs by BSA and further modification with PEI realized transfection using NRs into cultured cells. Moreover, the cellular uptake of NRs enabled cellular imaging using light scattering from the NRs.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalJournal of Nanoparticle Research
Volume10
Issue number1
DOIs
Publication statusPublished - Feb 1 2008

Fingerprint

Surface Modification
Nanorods
Gold
nanorods
Surface treatment
gold
Polyethyleneimine
Bovine Serum Albumin
albumins
serums
Absorption Spectroscopy
Absorption spectroscopy
Aggregation
absorption spectroscopy
Agglomeration
Cultured Cells
Gold Nanoparticles
gene expression
Light Scattering
Transmission Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Atomic and Molecular Physics, and Optics
  • Modelling and Simulation
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Surface modification of gold nanorods using layer-by-layer technique for cellular uptake. / Takahashi, Hironobu; Niidome, Takuro; Kawano, Takahito; Yamada, Sunao; Niidome, Yasuro.

In: Journal of Nanoparticle Research, Vol. 10, No. 1, 01.02.2008, p. 221-228.

Research output: Contribution to journalArticle

Takahashi, Hironobu ; Niidome, Takuro ; Kawano, Takahito ; Yamada, Sunao ; Niidome, Yasuro. / Surface modification of gold nanorods using layer-by-layer technique for cellular uptake. In: Journal of Nanoparticle Research. 2008 ; Vol. 10, No. 1. pp. 221-228.
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