Surface modification of oriented polysaccharide scaffolds using biotic nanofibers for epidermal cell culture

Suresh N. Rao, Yutaro Tsujita, Tetsuo Kondo

Research output: Contribution to journalArticle

Abstract

In this study, a novel scaffold was fabricated from nematically ordered polysaccharide molecules such as cellulose, chitin and cellulose-chitin blend (50–50%), termed as NOC, NOCh, NOC-Ch, by deposition of biotic nanofibers such as bacterial cellulose (BC) and collagen, which were prepared by aqueous counter collision method to allow the fiber width to reduce into nano-scales by high pressure impinging of aqueous suspension jets. The resultant aqueous dispersion of the nanofibers was spread over the templates using the spin coating technique and its surface morphology, roughness, strength, and hydrophobicity were characterized by atomic force microscopy followed by the tensile strength and contact angle measurements. The results indicated that, after initial deposition of BC nanofibers, the surface wettability of the scaffolds switched from hydrophobic to hydrophilic, whereas for collagen nanofibers, the surface hydrophobicity was increased. Repeated coating also improved the strength of the scaffold. Cell culture studies were carried out to explore the potentials of the modified scaffolds using the normal human epidermal cells. The results showed that the number of nanofiber coating cycles and surface morphology on the scaffolds played a role in cell elongation and its morphology. Particularly in this study, we have focussed on the changes in the morphogenesis such as cell adhesion and cell size grown on 1, 3 and 5 times coated scaffolds. The result indicated that the morphological changes depending on the coating nanofibers in the surface of scaffolds were related to increase in the epidermal cell size and altering cell adhesion.

Original languageEnglish
Pages (from-to)7971-7981
Number of pages11
JournalCellulose
Volume26
Issue number13-14
DOIs
Publication statusPublished - Sep 30 2019

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Scaffolds (biology)
Polysaccharides
Nanofibers
Cell culture
Surface treatment
Scaffolds
Cellulose
Chitin
Cell adhesion
Hydrophobicity
Collagen
Coatings
Surface morphology
Coating techniques
Spin coating
Angle measurement
Contact angle
Wetting
Elongation
Atomic force microscopy

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

Cite this

Surface modification of oriented polysaccharide scaffolds using biotic nanofibers for epidermal cell culture. / Rao, Suresh N.; Tsujita, Yutaro; Kondo, Tetsuo.

In: Cellulose, Vol. 26, No. 13-14, 30.09.2019, p. 7971-7981.

Research output: Contribution to journalArticle

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