Abstract
The relationship between surface marker expression and encephalitogenicity of spleen cells was studied in Lewis rats. Donors were sensitized with either BP/CFA or BP/IFA, or given BP-cultured naive spleen cells. The adoptive transfer of EAE was successfully achieved in every case after culture with BP, although only spleen cells from BP/CFA-sensitized rats proliferated significantly in response to BP. The observed encephalitogenicities were BP/CFA-sensitized cells > BP/IFA-sensitized cells ≫ cells from recipients of BP-cultured naive cells in descending order. In BP/CFA-sensitized cells, the expression of both W3/25 and OX-3 antigens on T cells increased markedly after culture with BP, but the expression of neither OX-19 nor OX-8 antigen increased significantly. Cells from neither BP/IFA-sensitized rats nor recipients of BP-cultured naive cells showed a significant change in the surface marker expression after culture with BP. Therefore, the generation of T cells coexpressing large amounts of both W3/25 and OX-3 antigens after culture with BP seems to correspond to the acquisition of the strong encephalitogenicity in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 365-379 |
| Number of pages | 15 |
| Journal | Journal of Neuroimmunology |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 1987 |
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology