Surface plasmon field-enhanced fluorescence spectroscopy apparatus with a convergent optical system for point-of-care testing

Mitsuaki Toda, Yusuke Arima, Hiromi Takiguchi, Hiroo Iwata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) is a promising methodology for point-of-care (POC) testing. The SPFS devices that have been reported are equipped with an angle rotating stage to adjust the surface plasmon resonance (SPR) angle. In a clinical setting, however, the SPR angle determination is a tedious and time-consuming process. In this study, we employed an SPFS instrument with a convergent optical system that allows the omission of this procedure. We demonstrated that this instrumentation allowed the sensitive determination of low concentrations of α-fetoprotein in serum and reduced the variation effect caused by the protein concentrations in samples. The SPFS with a convergent optical system is suitable for POC testing.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalAnalytical Biochemistry
Volume467
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Optical Devices
Fluorescence Spectrometry
Fluorescence spectroscopy
Optical systems
Surface Plasmon Resonance
Surface plasmon resonance
Testing
Fetal Proteins
Equipment and Supplies
Point-of-Care Testing
Serum
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Surface plasmon field-enhanced fluorescence spectroscopy apparatus with a convergent optical system for point-of-care testing. / Toda, Mitsuaki; Arima, Yusuke; Takiguchi, Hiromi; Iwata, Hiroo.

In: Analytical Biochemistry, Vol. 467, 01.01.2014, p. 47-53.

Research output: Contribution to journalArticle

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