Surface plasmon resonance-based detection of ladder-shaped polyethers by inhibition detection method

Ryota Mouri, Tohru Oishi, Kohei Torikai, Satoru Ujihara, Nobuaki Matsumori, Michio Murata, Yasukatsu Oshima

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ladder-shaped polyether (LSP) compounds represented by brevetoxins and ciguatoxins were largely discovered in association with seafood poisoning. Thus, a quick quantification method for LSPs is potentially important. We examined a surface plasmon resonance method using desulfated-yessotoxin (dsYTX) immobilized on a sensor chip and phosphodiesterase PDEII in a inhibition detection mode. Yessotoxin, brevetoxin B and synthetic LSP derivatives showed clear inhibition against PDEII binding to the immobilized dsYTX, by which their half inhibitory concentrations were successfully estimated. This inhibition method appeared to be superior in specificity to direct binding assays where binding proteins to LSP was immobilized on a sensor chip.

Original languageEnglish
Pages (from-to)2824-2828
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number10
DOIs
Publication statusPublished - May 15 2009

Fingerprint

Surface Plasmon Resonance
Polyethers
Ladders
Surface plasmon resonance
Ciguatoxins
Seafood
Sensors
Phosphoric Diester Hydrolases
Poisoning
Assays
Carrier Proteins
Association reactions
Derivatives
yessotoxin

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Surface plasmon resonance-based detection of ladder-shaped polyethers by inhibition detection method. / Mouri, Ryota; Oishi, Tohru; Torikai, Kohei; Ujihara, Satoru; Matsumori, Nobuaki; Murata, Michio; Oshima, Yasukatsu.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 19, No. 10, 15.05.2009, p. 2824-2828.

Research output: Contribution to journalArticle

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