Surface plasmon resonance based sensitive immunosensor for benzaldehyde detection

Takeshi Onodera, Takuzo Shimizu, Norio Miura, Kiyoshi Matsumoto, Kiyoshi Toko

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Fragrant compounds used to add flavor to beverages remain in the manufacturing line after the beverage manufacturing process. Line cleanliness before the next manufacturing cycle is difficult to estimate by sensory analysis, making excessive washing necessary. A new measurement system to determine line cleanliness is desired. In this study, we attempted to detect benzaldehyde (Bz) using an anti-Bz monoclonal antibody (Bz-Ab) and a surface plasmon resonance (SPR) sensor. We fabricated two types of sensor chips using self-assembled monolayers (SAMs) and investigated which sensor surface exhibited higher sensitivity. In addition, anti-Bz antibody conjugated with horseradish peroxidase (HRP-Bz-Ab) was used to enhance the SPR signal. A detection limit of co. 9ng/mL (ppb) was achieved using an immobilized 4-carboxybenzaldehyde sensor surface using SAMs containing ethylene glycol. When the HRP-Bz-Ab concentration was reduced to 30ng/mL, a detection limit of co. 4ng/mL (ppb) was achieved for Bz.

Original languageEnglish
JournalIEEJ Transactions on Sensors and Micromachines
Volume130
Issue number7
DOIs
Publication statusPublished - Aug 18 2010

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this