Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays

Yusuke Inoue, Takeshi Mori, Go Yamanouchi, Xiaoming Han, Tatsuhiko Sonoda, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Enzymatic activity monitoring of caspases, which are a class of cysteine protease, was performed by using peptide arrays based on surface plasmon resonance (SPR) imaging. The strategy of the detection is straightforward, using streptavidin to amplify the SPR signals of the surface-immobilized substrate peptides labeled with biotin at the C termini. Thus, the cleavage of the substrate peptides by caspases was detected as a signal decrease. Using this method, we succeeded in monitoring the activities of purified caspases and caspases in cell lysates. The SPR imaging-based peptide array would be applicable to cell-based drug screening and biochemical studies to reveal signal transduction processes.

Original languageEnglish
Pages (from-to)147-149
Number of pages3
JournalAnalytical Biochemistry
Volume375
Issue number1
DOIs
Publication statusPublished - Apr 1 2008

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Surface Plasmon Resonance
Surface plasmon resonance
Microarrays
Caspases
Imaging techniques
Peptides
Signal transduction
Preclinical Drug Evaluations
Streptavidin
Cysteine Proteases
Monitoring
Substrates
Biotin
Signal Transduction
Screening
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays. / Inoue, Yusuke; Mori, Takeshi; Yamanouchi, Go; Han, Xiaoming; Sonoda, Tatsuhiko; Niidome, Takuro; Katayama, Yoshiki.

In: Analytical Biochemistry, Vol. 375, No. 1, 01.04.2008, p. 147-149.

Research output: Contribution to journalArticle

Inoue, Yusuke ; Mori, Takeshi ; Yamanouchi, Go ; Han, Xiaoming ; Sonoda, Tatsuhiko ; Niidome, Takuro ; Katayama, Yoshiki. / Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays. In: Analytical Biochemistry. 2008 ; Vol. 375, No. 1. pp. 147-149.
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