Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction

Yan Li, Masatoshi Kobayashi, Koji Furui, Nobuaki Soh, Koji Nakano, Toshihiko Imato

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)

    Abstract

    The use of a surface plasmon resonance immunosensor for the analysis of histamine (β-imidazole ethylamine) is described. The method is based on an indirect competitive reaction of an anti-histamine antibody in a sample solution with histamine immobilized on a sensor chip and with histamine in the sample solution. A sensor chip immobilized with histamine was prepared using a self-assembly monolayer of 11-mercaptoundecanoic acid (11-MUA) as an anchor membrane, followed by an amino-coupling reaction with histamine after activation of the 11-MUA layer on the sensor chip by treatment with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide. The sensor chip can be reused, after regeneration with a 10 mM HCl solution, which dissociates the anti-histamine antibody complex from histamine on the sensor chip. The affinity constants for the immunocomplex of the anti-histamine antibody with histamine in the solution and for that of the anti-histamine antibody with histamine immobilized on the sensor chip were calculated to be 1.5 × 107 and 7.2 × 105 M-1, respectively, by assuming a Langmuir-type adsorption of the anti-histamine antibody to histamine immobilized on the sensor chip. The detection limit of the method was determined to be 3 ppb.

    Original languageEnglish
    Pages (from-to)77-83
    Number of pages7
    JournalAnalytica Chimica Acta
    Volume576
    Issue number1
    DOIs
    Publication statusPublished - Aug 18 2006

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Analytical Chemistry
    • Spectroscopy
    • Environmental Chemistry

    Fingerprint

    Dive into the research topics of 'Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction'. Together they form a unique fingerprint.

    Cite this