Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT

Toshikazu Kawaguchi, Dhesingh Ravi Shankaran, Sook Jin Kim, Kiyoshi Matsumoto, Kiyoshi Toko, Norio Miura

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

In order to develop the fully integrated portable surface plasmon resonance (SPR) system for detection of explosives, the amplification strategy of SPR signal was investigated. Indirect competitive inhibition method allowed the middle-sized SPR sensor to detect trinitrotoluene (TNT) at ppt level. However, this enhanced SPR signal was not high enough to detect TNT at ppt level by a miniaturized SPR sensor. Therefore, localized surface plasmon resonance (LSPR) effect using Au nanoparticle as further signal amplification approach was used. The amplification method of indirect competitive inhibition and LSPR were combined together for fabrication of the immunosurface using Au nanoparticle. TNT detectable range of this immunosurface was from 10 ppt (10 pg/ml) to 100 ppb (100 ng/ml), which was almost comparable to that without Au nanoparticle. The observed resonance angle change due to binding monoclonal TNT antibody (M-TNT Ab) with the immunosurface modified with Au nanoparticle was amplified to four times higher than that in absence of Au nanoparticle.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume133
Issue number2
DOIs
Publication statusPublished - Aug 12 2008

Fingerprint

Trinitrotoluene
Immunosensors
trinitrotoluene
Surface plasmon resonance
surface plasmon resonance
Nanoparticles
nanoparticles
Amplification
Monoclonal antibodies
sensors
Sensors
antibodies
Fabrication
fabrication

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

Cite this

Kawaguchi, T., Shankaran, D. R., Kim, S. J., Matsumoto, K., Toko, K., & Miura, N. (2008). Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT. Sensors and Actuators, B: Chemical, 133(2), 467-472. https://doi.org/10.1016/j.snb.2008.03.005

Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT. / Kawaguchi, Toshikazu; Shankaran, Dhesingh Ravi; Kim, Sook Jin; Matsumoto, Kiyoshi; Toko, Kiyoshi; Miura, Norio.

In: Sensors and Actuators, B: Chemical, Vol. 133, No. 2, 12.08.2008, p. 467-472.

Research output: Contribution to journalArticle

Kawaguchi, T, Shankaran, DR, Kim, SJ, Matsumoto, K, Toko, K & Miura, N 2008, 'Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT', Sensors and Actuators, B: Chemical, vol. 133, no. 2, pp. 467-472. https://doi.org/10.1016/j.snb.2008.03.005
Kawaguchi, Toshikazu ; Shankaran, Dhesingh Ravi ; Kim, Sook Jin ; Matsumoto, Kiyoshi ; Toko, Kiyoshi ; Miura, Norio. / Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT. In: Sensors and Actuators, B: Chemical. 2008 ; Vol. 133, No. 2. pp. 467-472.
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