Abstract
In order to develop the fully integrated portable surface plasmon resonance (SPR) system for detection of explosives, the amplification strategy of SPR signal was investigated. Indirect competitive inhibition method allowed the middle-sized SPR sensor to detect trinitrotoluene (TNT) at ppt level. However, this enhanced SPR signal was not high enough to detect TNT at ppt level by a miniaturized SPR sensor. Therefore, localized surface plasmon resonance (LSPR) effect using Au nanoparticle as further signal amplification approach was used. The amplification method of indirect competitive inhibition and LSPR were combined together for fabrication of the immunosurface using Au nanoparticle. TNT detectable range of this immunosurface was from 10 ppt (10 pg/ml) to 100 ppb (100 ng/ml), which was almost comparable to that without Au nanoparticle. The observed resonance angle change due to binding monoclonal TNT antibody (M-TNT Ab) with the immunosurface modified with Au nanoparticle was amplified to four times higher than that in absence of Au nanoparticle.
| Original language | English |
|---|---|
| Pages (from-to) | 467-472 |
| Number of pages | 6 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 133 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 12 2008 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Electrochemistry
- Electrical and Electronic Engineering
Cite this
Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT. / Kawaguchi, Toshikazu; Shankaran, Dhesingh Ravi; Kim, Sook Jin; Matsumoto, Kiyoshi; Toko, Kiyoshi; Miura, Norio.
In: Sensors and Actuators, B: Chemical, Vol. 133, No. 2, 12.08.2008, p. 467-472.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT
AU - Kawaguchi, Toshikazu
AU - Shankaran, Dhesingh Ravi
AU - Kim, Sook Jin
AU - Matsumoto, Kiyoshi
AU - Toko, Kiyoshi
AU - Miura, Norio
PY - 2008/8/12
Y1 - 2008/8/12
N2 - In order to develop the fully integrated portable surface plasmon resonance (SPR) system for detection of explosives, the amplification strategy of SPR signal was investigated. Indirect competitive inhibition method allowed the middle-sized SPR sensor to detect trinitrotoluene (TNT) at ppt level. However, this enhanced SPR signal was not high enough to detect TNT at ppt level by a miniaturized SPR sensor. Therefore, localized surface plasmon resonance (LSPR) effect using Au nanoparticle as further signal amplification approach was used. The amplification method of indirect competitive inhibition and LSPR were combined together for fabrication of the immunosurface using Au nanoparticle. TNT detectable range of this immunosurface was from 10 ppt (10 pg/ml) to 100 ppb (100 ng/ml), which was almost comparable to that without Au nanoparticle. The observed resonance angle change due to binding monoclonal TNT antibody (M-TNT Ab) with the immunosurface modified with Au nanoparticle was amplified to four times higher than that in absence of Au nanoparticle.
AB - In order to develop the fully integrated portable surface plasmon resonance (SPR) system for detection of explosives, the amplification strategy of SPR signal was investigated. Indirect competitive inhibition method allowed the middle-sized SPR sensor to detect trinitrotoluene (TNT) at ppt level. However, this enhanced SPR signal was not high enough to detect TNT at ppt level by a miniaturized SPR sensor. Therefore, localized surface plasmon resonance (LSPR) effect using Au nanoparticle as further signal amplification approach was used. The amplification method of indirect competitive inhibition and LSPR were combined together for fabrication of the immunosurface using Au nanoparticle. TNT detectable range of this immunosurface was from 10 ppt (10 pg/ml) to 100 ppb (100 ng/ml), which was almost comparable to that without Au nanoparticle. The observed resonance angle change due to binding monoclonal TNT antibody (M-TNT Ab) with the immunosurface modified with Au nanoparticle was amplified to four times higher than that in absence of Au nanoparticle.
UR - http://www.scopus.com/inward/record.url?scp=47649097146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47649097146&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2008.03.005
DO - 10.1016/j.snb.2008.03.005
M3 - Article
VL - 133
SP - 467
EP - 472
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 2
ER -