Surface structure and biocompatibility of demineralized dentin matrix granules soaked in a simulated body fluid

Toshiyuki Akazawa, Masaru Murata, Jun Hino, Futami Nagano, Tatsuhiro Shigyo, Takafumi Nomura, Hiroyuki Inano, Kohji Itabashi, Tohru Yamagishi, Katsuo Nakamura, Touru Takahashi, Shunji Iida, Haruhiko Kashiwazaki

Research output: Contribution to journalArticle

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Abstract

Demineralized dentin matrix (DDM) granules with excellent biocompatibility were easily prepared using unnecessary human teeth by a new cooling-pulverizing and demineralizing technique. Extracted human teeth were pulverized together with saline ice at 12,000 rpm-rotation number of a ZrO 2 blade for 30 s in a ZrO 2 vessel. The pulverized granules exhibited the particle size distribution of 0.5-2 mm that was efficient for regeneration of alveolar bone. The (Ca/P) ratios of the granules were 1.60-1.66, which were close to the stoichiometric value of 1.67 for standard hydroxyapatite (HAp). Small amounts of Na + and Mg 2+ ions present at less than 1% were detected. The pulverized granules were dissolved with stirring under 500 rpm for 10-60 min in 2.0%-HNO 3 solutions to obtain partial or complete DDM granules. As the dissolution time increased, crystallinity of HAp phase lowered and asperity on surfaces of the granules became outstanding due to elution of mineral components. At the dissolution of 60 min, the pulverizing granules were completely demineralized and the weight decreased to about one-fifth. To improve surface activity of the DDM granules without denaturation of bone growth factors, the DDM granules were soaked at 309.5 K and pH 7.40 in a simulated body fluid (SBF). HAp microcrystals were gradually precipitated on surfaces of the DDM granules with increasing the soaking time. Different morphology of the precipitates was observed, depending on the demineralization situation of the pulverized granules. For the DDM with low dissolution efficiency of 42%, porous bone-like apatites at 24 h after the soaking and fiber-oriented aggregates at 144 h were recognized. The bioactive DDM granules were implanted into the subcutaneous tissues of the back region of rats. At 4 weeks after the implantation, bio-absorption by comparatively small amounts of multi-giant cells was recognized around the surface layers of DDM granules.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalApplied Surface Science
Volume262
DOIs
Publication statusPublished - Dec 1 2012
Externally publishedYes

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Body fluids
Biocompatibility
Surface structure
Hydroxyapatite
Bone
Dissolution
Durapatite
Microcrystals
Denaturation
Apatite
Particle size analysis
Ice
Rats
Precipitates
Minerals
Tissue
Cooling
Apatites
Demineralized Dentin Matrix
Fibers

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

Cite this

Surface structure and biocompatibility of demineralized dentin matrix granules soaked in a simulated body fluid. / Akazawa, Toshiyuki; Murata, Masaru; Hino, Jun; Nagano, Futami; Shigyo, Tatsuhiro; Nomura, Takafumi; Inano, Hiroyuki; Itabashi, Kohji; Yamagishi, Tohru; Nakamura, Katsuo; Takahashi, Touru; Iida, Shunji; Kashiwazaki, Haruhiko.

In: Applied Surface Science, Vol. 262, 01.12.2012, p. 51-55.

Research output: Contribution to journalArticle

Akazawa, T, Murata, M, Hino, J, Nagano, F, Shigyo, T, Nomura, T, Inano, H, Itabashi, K, Yamagishi, T, Nakamura, K, Takahashi, T, Iida, S & Kashiwazaki, H 2012, 'Surface structure and biocompatibility of demineralized dentin matrix granules soaked in a simulated body fluid', Applied Surface Science, vol. 262, pp. 51-55. https://doi.org/10.1016/j.apsusc.2012.01.053
Akazawa, Toshiyuki ; Murata, Masaru ; Hino, Jun ; Nagano, Futami ; Shigyo, Tatsuhiro ; Nomura, Takafumi ; Inano, Hiroyuki ; Itabashi, Kohji ; Yamagishi, Tohru ; Nakamura, Katsuo ; Takahashi, Touru ; Iida, Shunji ; Kashiwazaki, Haruhiko. / Surface structure and biocompatibility of demineralized dentin matrix granules soaked in a simulated body fluid. In: Applied Surface Science. 2012 ; Vol. 262. pp. 51-55.
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