Surfactant-histidine-heme ternary complex as a simple artificial heme enzyme in organic media

Noriho Kamiya, Masahiro Goto, Shintaro Furusaki

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A surfactant-heme complex which shows peroxidase activity in organic media has been prepared by a method utilizing water-in-oil (W/O) emulsions. Both the aqueous phase pH and the type of surfactant appeared to have prominent effect on the catalytic activity of the heme complex in benzene. The catalytic efficiency of the heme complex was enhanced more than ten times by adding histidine to the aqueous phase of W/O emulsions in the preparation process. The enhancement of peroxidase activity was observed only in a nonaqueous medium due to the increase of the effective concentration of histidine as an activator. In the present study, we propose a simple preparation method for an artificial heme enzyme which works in nonaqueous media.

Original languageEnglish
Pages (from-to)502-506
Number of pages5
JournalBiotechnology and Bioengineering
Volume64
Issue number4
DOIs
Publication statusPublished - Aug 20 1999

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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