Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26

Shimpei Nishikawa, Masamitsu Konno, Atsushi Hamabe, Shinichiro Hasegawa, Yoshihiro Kano, Takahito Fukusumi, Taroh Satoh, Shuji Takiguchi, Masaki Mori, Yuichiro Doki, Hideshi Ishii

Research output: Contribution to journalLetter

20 Citations (Scopus)

Abstract

Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the present study, an expression profiling study was performed in which an anti‑cell surface marker antibody‑based array platform, a flow cytometry‑based cell separation technique and a tumorigenicity analysis in immunodeficient animals were utilized. These approaches revealed that the markers cluster of differentiation (CD)44 and CD26 facilitated the fractionation of surgically resected human gastric cancer (GC) cells into the following subset populations with distinct tumorigenic potentials: Highly tumorigenic CD26 + CD44 + cells (6/6 mice formed tumors), moderately tumorigenic CD26 + CD44 cells (5/6 mice formed tumors), and weakly or non‑tumorigenic CD26 CD44 cells (2/6 mice formed tumors). Furthermore, exposure to 5‑fluorouracil significantly increased the proportion of CD26 + cells in vitro. The present study demonstrated that the combined expression of CD26 and CD44 presents a potential marker of human GC stem cells.

Original languageEnglish
Pages (from-to)2361-2367
Number of pages7
JournalOncology Letters
Volume9
Issue number5
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Neoplastic Stem Cells
Stomach Neoplasms
Neoplasms
CD Antigens
Cell Separation
Population
Neoplasm Metastasis
Recurrence
Drug Therapy

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Nishikawa, S., Konno, M., Hamabe, A., Hasegawa, S., Kano, Y., Fukusumi, T., ... Ishii, H. (2015). Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26. Oncology Letters, 9(5), 2361-2367. https://doi.org/10.3892/ol.2015.3063

Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26. / Nishikawa, Shimpei; Konno, Masamitsu; Hamabe, Atsushi; Hasegawa, Shinichiro; Kano, Yoshihiro; Fukusumi, Takahito; Satoh, Taroh; Takiguchi, Shuji; Mori, Masaki; Doki, Yuichiro; Ishii, Hideshi.

In: Oncology Letters, Vol. 9, No. 5, 01.01.2015, p. 2361-2367.

Research output: Contribution to journalLetter

Nishikawa, S, Konno, M, Hamabe, A, Hasegawa, S, Kano, Y, Fukusumi, T, Satoh, T, Takiguchi, S, Mori, M, Doki, Y & Ishii, H 2015, 'Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26', Oncology Letters, vol. 9, no. 5, pp. 2361-2367. https://doi.org/10.3892/ol.2015.3063
Nishikawa, Shimpei ; Konno, Masamitsu ; Hamabe, Atsushi ; Hasegawa, Shinichiro ; Kano, Yoshihiro ; Fukusumi, Takahito ; Satoh, Taroh ; Takiguchi, Shuji ; Mori, Masaki ; Doki, Yuichiro ; Ishii, Hideshi. / Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26. In: Oncology Letters. 2015 ; Vol. 9, No. 5. pp. 2361-2367.
@article{a7f62e843d6543abaa057d342590f8c4,
title = "Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26",
abstract = "Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the present study, an expression profiling study was performed in which an anti‑cell surface marker antibody‑based array platform, a flow cytometry‑based cell separation technique and a tumorigenicity analysis in immunodeficient animals were utilized. These approaches revealed that the markers cluster of differentiation (CD)44 and CD26 facilitated the fractionation of surgically resected human gastric cancer (GC) cells into the following subset populations with distinct tumorigenic potentials: Highly tumorigenic CD26 + CD44 + cells (6/6 mice formed tumors), moderately tumorigenic CD26 + CD44 ‑ cells (5/6 mice formed tumors), and weakly or non‑tumorigenic CD26 ‑ CD44 ‑ cells (2/6 mice formed tumors). Furthermore, exposure to 5‑fluorouracil significantly increased the proportion of CD26 + cells in vitro. The present study demonstrated that the combined expression of CD26 and CD44 presents a potential marker of human GC stem cells.",
author = "Shimpei Nishikawa and Masamitsu Konno and Atsushi Hamabe and Shinichiro Hasegawa and Yoshihiro Kano and Takahito Fukusumi and Taroh Satoh and Shuji Takiguchi and Masaki Mori and Yuichiro Doki and Hideshi Ishii",
year = "2015",
month = "1",
day = "1",
doi = "10.3892/ol.2015.3063",
language = "English",
volume = "9",
pages = "2361--2367",
journal = "Oncology Letters",
issn = "1792-1074",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26

AU - Nishikawa, Shimpei

AU - Konno, Masamitsu

AU - Hamabe, Atsushi

AU - Hasegawa, Shinichiro

AU - Kano, Yoshihiro

AU - Fukusumi, Takahito

AU - Satoh, Taroh

AU - Takiguchi, Shuji

AU - Mori, Masaki

AU - Doki, Yuichiro

AU - Ishii, Hideshi

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the present study, an expression profiling study was performed in which an anti‑cell surface marker antibody‑based array platform, a flow cytometry‑based cell separation technique and a tumorigenicity analysis in immunodeficient animals were utilized. These approaches revealed that the markers cluster of differentiation (CD)44 and CD26 facilitated the fractionation of surgically resected human gastric cancer (GC) cells into the following subset populations with distinct tumorigenic potentials: Highly tumorigenic CD26 + CD44 + cells (6/6 mice formed tumors), moderately tumorigenic CD26 + CD44 ‑ cells (5/6 mice formed tumors), and weakly or non‑tumorigenic CD26 ‑ CD44 ‑ cells (2/6 mice formed tumors). Furthermore, exposure to 5‑fluorouracil significantly increased the proportion of CD26 + cells in vitro. The present study demonstrated that the combined expression of CD26 and CD44 presents a potential marker of human GC stem cells.

AB - Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the present study, an expression profiling study was performed in which an anti‑cell surface marker antibody‑based array platform, a flow cytometry‑based cell separation technique and a tumorigenicity analysis in immunodeficient animals were utilized. These approaches revealed that the markers cluster of differentiation (CD)44 and CD26 facilitated the fractionation of surgically resected human gastric cancer (GC) cells into the following subset populations with distinct tumorigenic potentials: Highly tumorigenic CD26 + CD44 + cells (6/6 mice formed tumors), moderately tumorigenic CD26 + CD44 ‑ cells (5/6 mice formed tumors), and weakly or non‑tumorigenic CD26 ‑ CD44 ‑ cells (2/6 mice formed tumors). Furthermore, exposure to 5‑fluorouracil significantly increased the proportion of CD26 + cells in vitro. The present study demonstrated that the combined expression of CD26 and CD44 presents a potential marker of human GC stem cells.

UR - http://www.scopus.com/inward/record.url?scp=84925437200&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925437200&partnerID=8YFLogxK

U2 - 10.3892/ol.2015.3063

DO - 10.3892/ol.2015.3063

M3 - Letter

AN - SCOPUS:84925437200

VL - 9

SP - 2361

EP - 2367

JO - Oncology Letters

JF - Oncology Letters

SN - 1792-1074

IS - 5

ER -