Survey of solasodine-type glycoalkaloids by western blotting and ELISA using anti-solamargine monoclonal antibody

Waraporn Putalun, Hiroyuki Tanaka, Shoji Yahara, Sorasak Lhieochaiphan, Yukihiro Shoyama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Solasodine glycosides were analyzed for the selection of higher- producing natural resources in the fruits of Solanum spp. by western blotting and ELISA using anti-solamargine monoclonal antibody (MAb). Western blotting of Solanum khasianum showed a correlation with the analysis by ELISA. The results indicated that the fruits of S. khasianum contained the highest level of solasodine glycosides, compared to other species having 53.43±3.90 μg/mg dry wt. The high level of solasodine glycosides in S. khasianum suggests that it may be suitable for commercial production.

Original languageEnglish
Pages (from-to)72-75
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

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Glycosides
Solanum
Western Blotting
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Fruit
solasodine
Surveys and Questionnaires
beta-solamarine

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Survey of solasodine-type glycoalkaloids by western blotting and ELISA using anti-solamargine monoclonal antibody. / Putalun, Waraporn; Tanaka, Hiroyuki; Yahara, Shoji; Lhieochaiphan, Sorasak; Shoyama, Yukihiro.

In: Biological and Pharmaceutical Bulletin, Vol. 23, No. 1, 01.01.2000, p. 72-75.

Research output: Contribution to journalArticle

Putalun, Waraporn ; Tanaka, Hiroyuki ; Yahara, Shoji ; Lhieochaiphan, Sorasak ; Shoyama, Yukihiro. / Survey of solasodine-type glycoalkaloids by western blotting and ELISA using anti-solamargine monoclonal antibody. In: Biological and Pharmaceutical Bulletin. 2000 ; Vol. 23, No. 1. pp. 72-75.
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