TY - JOUR
T1 - Survivability and subsequent development of vitrified early-stage mouse embryos after warming at different temperatures
AU - Taketsuru, Hiroaki
AU - Tsukada, Yu ichi
AU - Kaneko, Takehito
N1 - Funding Information:
This work was supported by NIBB Collaborative Research Program ( 17-909 , 18–903 , 19–907 ) to T.K, Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science ( 20H00420 ), and the Environment Research and Technology Development Fund of the Environmental Restoration and Conservation Agency of Japan ( 4-2101 ).
Funding Information:
This work was supported by NIBB Collaborative Research Program (17-909, 18?903, 19?907) to T.K, Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (20H00420), and the Environment Research and Technology Development Fund of the Environmental Restoration and Conservation Agency of Japan (4-2101).
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2022/2/5
Y1 - 2022/2/5
N2 - Cryopreservation of embryos is a useful method for stably preserving various strains for a long time, and the cryopreserved embryos can be used at any time by simple warming. However, the viability of cryopreserved embryos, particularly vitrification at an early stage, is low compared to that of fresh embryos. As the warming process during vitrification is known to affect the survivability and subsequent development of embryos, the present study aimed to examine the viability and subsequent development of vitrified early-stage mouse embryos after warming at different temperatures. The survival rate of pronuclear and 2-cell stage embryos warmed at 60 °C (97% and 88%, respectively) was significantly higher than that of the embryos warmed at 37 °C (46% and 48%, respectively). The pronuclear and 2-cell stage embryos warmed at 60 °C (86% and 100%) showed better development to the blastocyst stage than the embryos warmed at 37 °C (72% and 84%, respectively). The development of offspring of the surviving embryos was similar at both the warming temperatures. These results showed that the survivability and subsequent development of vitrified early-stage mouse embryos were obviously increased upon rapid warming. This improved warming process could be helpful for the maintenance and reproduction of genetic resources.
AB - Cryopreservation of embryos is a useful method for stably preserving various strains for a long time, and the cryopreserved embryos can be used at any time by simple warming. However, the viability of cryopreserved embryos, particularly vitrification at an early stage, is low compared to that of fresh embryos. As the warming process during vitrification is known to affect the survivability and subsequent development of embryos, the present study aimed to examine the viability and subsequent development of vitrified early-stage mouse embryos after warming at different temperatures. The survival rate of pronuclear and 2-cell stage embryos warmed at 60 °C (97% and 88%, respectively) was significantly higher than that of the embryos warmed at 37 °C (46% and 48%, respectively). The pronuclear and 2-cell stage embryos warmed at 60 °C (86% and 100%) showed better development to the blastocyst stage than the embryos warmed at 37 °C (72% and 84%, respectively). The development of offspring of the surviving embryos was similar at both the warming temperatures. These results showed that the survivability and subsequent development of vitrified early-stage mouse embryos were obviously increased upon rapid warming. This improved warming process could be helpful for the maintenance and reproduction of genetic resources.
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U2 - 10.1016/j.bbrc.2021.12.092
DO - 10.1016/j.bbrc.2021.12.092
M3 - Article
C2 - 34999253
AN - SCOPUS:85122234395
VL - 591
SP - 50
EP - 53
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
ER -