TY - JOUR
T1 - Survival and differentiation of embryonic neural explants on different biomaterials
AU - Soria, José Miguel
AU - Martínez Ramos, Cristina
AU - Salmerón Sánchez, Manuel
AU - Benavent, Veronique
AU - Campillo Fernández, Alberto
AU - Gómez Ribelles, José Luis
AU - García Verdugo, José Manuel
AU - Monleón Pradas, Manuel
AU - Barcia, Juan Antonio
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture.
AB - Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture.
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U2 - 10.1002/jbm.a.30803
DO - 10.1002/jbm.a.30803
M3 - Article
C2 - 16788975
AN - SCOPUS:33751584212
VL - 79
SP - 495
EP - 502
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 3
ER -