Sustained βAR stimulation mediates cardiac insulin resistance in a PKA-dependent manner

Supachoke Mangmool, Tananat Denkaew, Sarawuth Phosri, Darawan Pinthong, Warisara Parichatikanond, Tsukasa Shimauchi, Motohiro Nishida

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Insulin resistance is a condition in which cells are defective in response to the actions of insulin in tissue glucose uptake. Overstimulation of β-adrenergic receptors (βARs) leads to the development of heart failure and is associated with the pathogenesis of insulin resistance in the heart. However, the mechanisms by which sustained βAR stimulation affects insulin resistance in the heart are incompletely understood. In this study, we demonstrate that sustained βAR stimulation resulted in the inhibition of insulin-induced glucose uptake, and a reduction of insulin induced glucose transporter (GLUT)4 expression that were mediated by the β2AR subtype in cardiomyocytes and heart tissue. Overstimulation of β2AR inhibited the insulin-induced translocation of GLUT4 to the plasma membrane of cardiomyocytes. Additionally, βAR mediated cardiac insulin resistance by reducing glucose uptake and GLUT4 expression via the cAMP-dependent and protein kinase A-dependent pathways. Treatment with β-blockers, including propranolol and metoprolol antagonized isoproterenol-mediated insulin resistance in the heart. The data in this present study confirm a critical role for protein kinase A in βAR-mediated insulin resistance.

Original languageEnglish
Pages (from-to)118-132
Number of pages15
JournalMolecular Endocrinology
Volume30
Issue number1
DOIs
Publication statusPublished - Jan 2016

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

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