Sympathetic activation of leptin via the ventromedial hypothalamus: Leptin-induced increase in catecholamine secretion

Noriko Satoh, Yoshihiro Ogawa, Goro Katsuura, Yoshito Numata, Tetsuo Tsuji, Minoru Hayase, Ken Ebihara, Hiroaki Masuzaki, Kiminori Hosoda, Yasunao Yoshimasa, Kazuwa Nakao

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Leptin is an adipocyte-derived blood-borne satiety factor that acts directly on the hypothalamus, thereby regulating food intake and energy expenditure. We have demonstrated that the hypothalamic arcuate nucleus (Arc) is a primary site of the satiety effect of leptin (Neurosci Lett 224:149-152, 1997). To explore the hypothalamic pathway of sympathetic activation of leptin, we examined the effects of a single intravenous or intracerebroventricular injection of recombinant human leptin on catecholamine secretion in rats. We also examined the effects of direct microinjection of leptin into the ventromedial hypothalamus (VMH), Arc, paraventricular nucleus (PVN), and dorsomedial hypothalamus (DMH) in rats. To further assess whether sympathetic activation of leptin is mediated via the VMH, we also examined the effects of a single intravenous injection of leptin in VMH-lesioned rats. A single injection of leptin (0.25-1.0 mg i.v./rat or 0.5-2.0 pg i.c.v./rat) increased plasma norepinephrine (NE) and epinephrine (EPI) concentrations in a dose-dependent manner. Plasma NE and EPI concentrations were increased significantly when leptin was injected directly into the VMH but were unchanged when injected into the Arc, PVN, and DMH. Plasma NE and EPI concentrations were unchanged in VMH-lesioned rats that received a single intravenous injection of leptin. The present study provides evidence that a leptin-induced increase in catecholamine secretion is mediated primarily via the VMH and suggests the presence of distinct hypothalamic pathways mediating the satiety effect and sympathetic activation of leptin.

Original languageEnglish
Pages (from-to)1787-1793
Number of pages7
JournalDiabetes
Volume48
Issue number9
DOIs
Publication statusPublished - Sep 11 1999
Externally publishedYes

Fingerprint

Leptin
Hypothalamus
Catecholamines
Epinephrine
Norepinephrine
Paraventricular Hypothalamic Nucleus
Intravenous Injections
Arcuate Nucleus of Hypothalamus
Injections
Microinjections
Adipocytes
Energy Metabolism
Eating

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Sympathetic activation of leptin via the ventromedial hypothalamus : Leptin-induced increase in catecholamine secretion. / Satoh, Noriko; Ogawa, Yoshihiro; Katsuura, Goro; Numata, Yoshito; Tsuji, Tetsuo; Hayase, Minoru; Ebihara, Ken; Masuzaki, Hiroaki; Hosoda, Kiminori; Yoshimasa, Yasunao; Nakao, Kazuwa.

In: Diabetes, Vol. 48, No. 9, 11.09.1999, p. 1787-1793.

Research output: Contribution to journalArticle

Satoh, N, Ogawa, Y, Katsuura, G, Numata, Y, Tsuji, T, Hayase, M, Ebihara, K, Masuzaki, H, Hosoda, K, Yoshimasa, Y & Nakao, K 1999, 'Sympathetic activation of leptin via the ventromedial hypothalamus: Leptin-induced increase in catecholamine secretion', Diabetes, vol. 48, no. 9, pp. 1787-1793. https://doi.org/10.2337/diabetes.48.9.1787
Satoh, Noriko ; Ogawa, Yoshihiro ; Katsuura, Goro ; Numata, Yoshito ; Tsuji, Tetsuo ; Hayase, Minoru ; Ebihara, Ken ; Masuzaki, Hiroaki ; Hosoda, Kiminori ; Yoshimasa, Yasunao ; Nakao, Kazuwa. / Sympathetic activation of leptin via the ventromedial hypothalamus : Leptin-induced increase in catecholamine secretion. In: Diabetes. 1999 ; Vol. 48, No. 9. pp. 1787-1793.
@article{d28a07b76e3842dca5235a94110721ed,
title = "Sympathetic activation of leptin via the ventromedial hypothalamus: Leptin-induced increase in catecholamine secretion",
abstract = "Leptin is an adipocyte-derived blood-borne satiety factor that acts directly on the hypothalamus, thereby regulating food intake and energy expenditure. We have demonstrated that the hypothalamic arcuate nucleus (Arc) is a primary site of the satiety effect of leptin (Neurosci Lett 224:149-152, 1997). To explore the hypothalamic pathway of sympathetic activation of leptin, we examined the effects of a single intravenous or intracerebroventricular injection of recombinant human leptin on catecholamine secretion in rats. We also examined the effects of direct microinjection of leptin into the ventromedial hypothalamus (VMH), Arc, paraventricular nucleus (PVN), and dorsomedial hypothalamus (DMH) in rats. To further assess whether sympathetic activation of leptin is mediated via the VMH, we also examined the effects of a single intravenous injection of leptin in VMH-lesioned rats. A single injection of leptin (0.25-1.0 mg i.v./rat or 0.5-2.0 pg i.c.v./rat) increased plasma norepinephrine (NE) and epinephrine (EPI) concentrations in a dose-dependent manner. Plasma NE and EPI concentrations were increased significantly when leptin was injected directly into the VMH but were unchanged when injected into the Arc, PVN, and DMH. Plasma NE and EPI concentrations were unchanged in VMH-lesioned rats that received a single intravenous injection of leptin. The present study provides evidence that a leptin-induced increase in catecholamine secretion is mediated primarily via the VMH and suggests the presence of distinct hypothalamic pathways mediating the satiety effect and sympathetic activation of leptin.",
author = "Noriko Satoh and Yoshihiro Ogawa and Goro Katsuura and Yoshito Numata and Tetsuo Tsuji and Minoru Hayase and Ken Ebihara and Hiroaki Masuzaki and Kiminori Hosoda and Yasunao Yoshimasa and Kazuwa Nakao",
year = "1999",
month = "9",
day = "11",
doi = "10.2337/diabetes.48.9.1787",
language = "English",
volume = "48",
pages = "1787--1793",
journal = "Diabetes",
issn = "0012-1797",
publisher = "医学出版",
number = "9",

}

TY - JOUR

T1 - Sympathetic activation of leptin via the ventromedial hypothalamus

T2 - Leptin-induced increase in catecholamine secretion

AU - Satoh, Noriko

AU - Ogawa, Yoshihiro

AU - Katsuura, Goro

AU - Numata, Yoshito

AU - Tsuji, Tetsuo

AU - Hayase, Minoru

AU - Ebihara, Ken

AU - Masuzaki, Hiroaki

AU - Hosoda, Kiminori

AU - Yoshimasa, Yasunao

AU - Nakao, Kazuwa

PY - 1999/9/11

Y1 - 1999/9/11

N2 - Leptin is an adipocyte-derived blood-borne satiety factor that acts directly on the hypothalamus, thereby regulating food intake and energy expenditure. We have demonstrated that the hypothalamic arcuate nucleus (Arc) is a primary site of the satiety effect of leptin (Neurosci Lett 224:149-152, 1997). To explore the hypothalamic pathway of sympathetic activation of leptin, we examined the effects of a single intravenous or intracerebroventricular injection of recombinant human leptin on catecholamine secretion in rats. We also examined the effects of direct microinjection of leptin into the ventromedial hypothalamus (VMH), Arc, paraventricular nucleus (PVN), and dorsomedial hypothalamus (DMH) in rats. To further assess whether sympathetic activation of leptin is mediated via the VMH, we also examined the effects of a single intravenous injection of leptin in VMH-lesioned rats. A single injection of leptin (0.25-1.0 mg i.v./rat or 0.5-2.0 pg i.c.v./rat) increased plasma norepinephrine (NE) and epinephrine (EPI) concentrations in a dose-dependent manner. Plasma NE and EPI concentrations were increased significantly when leptin was injected directly into the VMH but were unchanged when injected into the Arc, PVN, and DMH. Plasma NE and EPI concentrations were unchanged in VMH-lesioned rats that received a single intravenous injection of leptin. The present study provides evidence that a leptin-induced increase in catecholamine secretion is mediated primarily via the VMH and suggests the presence of distinct hypothalamic pathways mediating the satiety effect and sympathetic activation of leptin.

AB - Leptin is an adipocyte-derived blood-borne satiety factor that acts directly on the hypothalamus, thereby regulating food intake and energy expenditure. We have demonstrated that the hypothalamic arcuate nucleus (Arc) is a primary site of the satiety effect of leptin (Neurosci Lett 224:149-152, 1997). To explore the hypothalamic pathway of sympathetic activation of leptin, we examined the effects of a single intravenous or intracerebroventricular injection of recombinant human leptin on catecholamine secretion in rats. We also examined the effects of direct microinjection of leptin into the ventromedial hypothalamus (VMH), Arc, paraventricular nucleus (PVN), and dorsomedial hypothalamus (DMH) in rats. To further assess whether sympathetic activation of leptin is mediated via the VMH, we also examined the effects of a single intravenous injection of leptin in VMH-lesioned rats. A single injection of leptin (0.25-1.0 mg i.v./rat or 0.5-2.0 pg i.c.v./rat) increased plasma norepinephrine (NE) and epinephrine (EPI) concentrations in a dose-dependent manner. Plasma NE and EPI concentrations were increased significantly when leptin was injected directly into the VMH but were unchanged when injected into the Arc, PVN, and DMH. Plasma NE and EPI concentrations were unchanged in VMH-lesioned rats that received a single intravenous injection of leptin. The present study provides evidence that a leptin-induced increase in catecholamine secretion is mediated primarily via the VMH and suggests the presence of distinct hypothalamic pathways mediating the satiety effect and sympathetic activation of leptin.

UR - http://www.scopus.com/inward/record.url?scp=0032833029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032833029&partnerID=8YFLogxK

U2 - 10.2337/diabetes.48.9.1787

DO - 10.2337/diabetes.48.9.1787

M3 - Article

C2 - 10480609

AN - SCOPUS:0032833029

VL - 48

SP - 1787

EP - 1793

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 9

ER -