Sympathetic control of arterial membrane potential by ATP-sensitive K+- channels

Kenichi Goto, Koji Fujii, Isao Abe, Masatoshi Fujishima

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Stimulation of perivascular nerve terminals leads to a release of various neurotransmitters such as norepinephrine, epinephrine, acetylcholine, nitric oxide, and calcitonin gene-related peptide (CGRP). Because some of these substances have been shown to cause smooth muscle hyperpolarization by direct or endothelium-dependent mechanisms, we hypothesized that the liberation of I or more of these transmitters may lead to neurogenic hyperpolarization in arterial muscle cells. The present study was designed to determine the presence or absence of neurogenic hyperpolarization and, if present, its underlying mechanisms in isolated rat mesenteric resistance arteries, through the use of conventional microelectrode techniques. The experiments were performed under the combined blockade of α-adrenoceptors and purinoceptors with phentolamine and suramin to eliminate depolarizing responses to nerve stimulation. Under these conditions, perivascular nerve stimulation (5 Hz, 30 seconds) evoked smooth muscle hyperpolarization (-3.3 ± 0.3 mV, n = 15), which was abolished by tetrodotoxin, indicating the neurogenic origin of the response. This neurogenic hyperpolarization was resistant to atropine, nitro-L-arginine, or CGRP8-37, a CGRP antagonist, but was abolished by guanethidine and β-blocker propranolol. This hyperpolarization was also abolished by glibenclamide, an ATP-sensitive K+ channel (K(ATP)) blocker, but was unaffected by apamin, a Ca2+-activated K+ channel blocker. In separate experiments, exogenous norepinephrine caused glibenclamide-sensitive hyperpolarization in the presence of phentolamine. On the other hand, norepinephrine-induced depolarization in the absence of phentolamine was enhanced by propranolol. TheSe findings suggest that neurally released catecholamines cause membrane hyperpolarization through the activation of K(ATP) by β-adrenoceptors. Such hyperpolarization may play an important role in the control of arterial membrane potential by opposing α- adrenergic depolarization.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalHypertension
Volume35
Issue number1 II
Publication statusPublished - Jan 1 2000

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Phentolamine
Membrane Potentials
Norepinephrine
Calcitonin Gene-Related Peptide
Glyburide
Adenosine Triphosphate
Propranolol
Adrenergic Receptors
Smooth Muscle
Apamin
Purinergic Receptors
Calcium-Activated Potassium Channels
Guanethidine
Suramin
Mesenteric Arteries
Tetrodotoxin
Microelectrodes
Atropine
Adrenergic Agents
Muscle Cells

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Goto, K., Fujii, K., Abe, I., & Fujishima, M. (2000). Sympathetic control of arterial membrane potential by ATP-sensitive K+- channels. Hypertension, 35(1 II), 379-384.

Sympathetic control of arterial membrane potential by ATP-sensitive K+- channels. / Goto, Kenichi; Fujii, Koji; Abe, Isao; Fujishima, Masatoshi.

In: Hypertension, Vol. 35, No. 1 II, 01.01.2000, p. 379-384.

Research output: Contribution to journalArticle

Goto, K, Fujii, K, Abe, I & Fujishima, M 2000, 'Sympathetic control of arterial membrane potential by ATP-sensitive K+- channels', Hypertension, vol. 35, no. 1 II, pp. 379-384.
Goto, Kenichi ; Fujii, Koji ; Abe, Isao ; Fujishima, Masatoshi. / Sympathetic control of arterial membrane potential by ATP-sensitive K+- channels. In: Hypertension. 2000 ; Vol. 35, No. 1 II. pp. 379-384.
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