Synergism of staphylococcus aureus colonization and allergic reaction in the nasal cavity in mice

Nobusuke Hohchi, Koichi Hashida, Jun Ichi Ohkubo, Tetsuro Wakasugi, Takanori Mori, Khac Hung Nguyen, Etsushi Kuroda, Takako Ikeno, Hatsumi Taniguchi, Hideaki Suzuki

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Abstract

Background: The aim of this study is to investigate the reciprocal effect of Staphylococcus aureus colonization and allergic rhinitis in an allergy model of mice. Methods: BALB/c mice with intraperitoneal ovalbumin (OVA) sensitization and/or intranasal S. aureus inoculation were prepared. The following 4 groups were designed: an OVA-sensitized S. aureus-inoculated (AR-SA) group, an OVA-sensitized uninoculated (AR) group, a nonsensitized S. aureus-inoculated (SA) group, and a nonsensitized uninoculated (control) group. After intranasal OVA challenge, nasal lavage fluid, peripheral blood, and nasal mucosa were collected. Polymorphonuclear cells in the nasal lavage fluid were counted, serum OVA-specific IgE and IgG1 were measured by enzyme immunoassays, and IL-4, IL-5, and IFN-γ mRNAs in the nasal mucosa were assessed by quantitative real-time reverse transcription-PCR. The number of S. aureus in the nasal mucosa and lavage fluid was counted. Results: Both eosinophil and neutrophil counts were larger in the AR-SA group than in the other groups. Both IgE and IgG1 levels were higher in the AR and AR-SA groups than in the SA and control groups, and the IgG1 level was higher in the AR-SA group than in the AR group. The expression of IL-4 mRNA was higher in the AR-SA group than in the other groups, and the expression of IL-5 mRNA was higher in the AR-SA group than in the SA group. The AR-SA group showed higher counts of S. aureus in the nasal mucosa than the SA group. Conclusion: These results indicate the mutually potentiating effect of S. aureus colonization and allergic rhinitis.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume159
Issue number1
DOIs
Publication statusPublished - Aug 1 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Hohchi, N., Hashida, K., Ohkubo, J. I., Wakasugi, T., Mori, T., Nguyen, K. H., ... Suzuki, H. (2012). Synergism of staphylococcus aureus colonization and allergic reaction in the nasal cavity in mice. International Archives of Allergy and Immunology, 159(1), 33-40. https://doi.org/10.1159/000335200