Synergistic actions of disulfide-reducing agents on 1-methyladenine-induced oocyte maturation in starfish

Michiyasu Yoshikuni, Katsutoshi Ishikawa, Minoru Isobe, Toshio Goto, Yoshitaka Nagahama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Several disulfide-reducing agents, such as dithiothreitol, 2,3-dimercapto-1-propanol, cysteine ethyl ester, and cysteine methyl ester enhanced the effectiveness of 1-methyladenine (1-MeAde) to induce oocyte maturation in the starfish Asterina pectinifera. This enhancement occurred at relatively low concentrations at which these agents by themselves were ineffective in inducing oocyte maturation. The agents caused a marked (about twofold) increase in specific [1-3H]MeAde binding. The binding increased directly in relation to the potency of the agents in enhancing 1-MeAde action. Scatchard analysis indicated that dithiothreitol increased the Bmax without affecting the affinity of 1-MeAde binding. These results strongly suggest that disulfide-reducing agents enhance the maturational action of 1-MeAde by increasing the number of 1-MeAde binding sites in oocyte cortices.

Original languageEnglish
Pages (from-to)236-239
Number of pages4
JournalDevelopmental Biology
Volume128
Issue number1
DOIs
Publication statusPublished - Jul 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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