Synergistic antitumor effect of interferon-β with gemcitabine in interferon-α-non-responsive pancreatic cancer cells

Yoshito Tomimaru, Hidetoshi Eguchi, Hiroshi Wada, Akira Tomokuni, Shogo Kobayashi, Shigeru Marubashi, Yutaka Takeda, Masahiro Tanemura, Koji Umeshita, Masaki Mori, Yuichiro Doki, Hiroaki Nagano

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Interferon (IFN)-β is reported to have more potent antitumor effects than IFN-α. The aim of this study was to compare the synergistic antitumor activity of both IFNs when combined with gemcitabine on cultured pancreatic cancer cells expressing various levels of IFN receptor. The growth-inhibitory effects of IFN-α and IFN-β in combination with gemcitabine on three human pancreatic cancer cell lines (BxPC-3, MIAPaCa-2, Panc-1) were evaluated by MTT assay and isobolographic analysis. We also correlated their growth-inhibitory effects with the expression status of type I IFN receptor type 2 (IFNAR2). Western blot analysis indicated strong expression of IFNAR2 in BxPC-3 and MIAPaCa-2, but weak expression in Panc-1. The growth-inhibitory effect of gemcitabine was enhanced synergistically by IFN-α in BxPC-3 and MIAPaCa-2, but not in Panc-1. IFN-β exhibited more potent synergistic growth-inhibitory effects with gemcitabine in BxPC-3 and MIAPaCa-2 compared to IFN-α, and also synergistic enhancement in Panc-1. In conclusion, our results indicated that the growth-inhibitory effect of IFN-β with gemcitabine was synergistic not only in pancreatic cancer cells with strong expression of IFNAR2, but also in those with weak expression of IFNAR2.

Original languageEnglish
Pages (from-to)1237-1243
Number of pages7
JournalInternational journal of oncology
Volume38
Issue number5
DOIs
Publication statusPublished - May 2011

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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