Synergistic effects of CDDP and OK-432-stimulated PBMC-producing cytokine mixture on tumor cell growth: A possible role of apoptosis

H. Matsunaga, M. Katano, M. Nakamura, T. Saita, M. Mori

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The streptococcal preparation, OK-432, is known to induce several antitumor cytokines (cytokine mixture) such as IFN, TNF, and TGIF in human peripheral blood mononuclear cells (PBMC). In this paper, the effect of CDDP and OK-432 induced cytokine mixture on the induction of apoptosis in a human gastric carcinoma cell line, MK-1, was investigated. Apoptosis was determined by morphological features and DNA fragmentation analysis. The cytokine mixture induced apoptosis in MK-1 cells in a dose and time dependent manner. The combination of a subthreshold concentration of CDDP and the cytokine mixture induced both significant growth inhibition and significant DNA fragmentation in MK 1 cells, which indicates that the effects of CDDP and cytokine mixture are synergistic.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalBiotherapy
Volume11
Issue number2
Publication statusPublished - Jan 1 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Synergistic effects of CDDP and OK-432-stimulated PBMC-producing cytokine mixture on tumor cell growth: A possible role of apoptosis'. Together they form a unique fingerprint.

Cite this