Abstract
The streptococcal preparation, OK-432, is known to induce several antitumor cytokines (cytokine mixture) such as IFN, TNF, and TGIF in human peripheral blood mononuclear cells (PBMC). In this paper, the effect of CDDP and OK-432 induced cytokine mixture on the induction of apoptosis in a human gastric carcinoma cell line, MK-1, was investigated. Apoptosis was determined by morphological features and DNA fragmentation analysis. The cytokine mixture induced apoptosis in MK-1 cells in a dose and time dependent manner. The combination of a subthreshold concentration of CDDP and the cytokine mixture induced both significant growth inhibition and significant DNA fragmentation in MK 1 cells, which indicates that the effects of CDDP and cytokine mixture are synergistic.
Original language | English |
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Pages (from-to) | 175-180 |
Number of pages | 6 |
Journal | Biotherapy |
Volume | 11 |
Issue number | 2 |
Publication status | Published - Jan 1 1997 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research