A calixarene carboxylic acid derivative incorporating tert-octyl groups at the upper rim was found to be soluble in an aliphatic organic solvent. The extraction behavior of various nucleobases was investigated with the calixarene carboxylic acid derivative. In an isooctane system, neither calixarene nor D2EHPA were very effective at nucleobase extraction. However, addition of D2EHPA along with calixarene drastically enhanced the efficiency of adenine and cytosine extraction. It was found that D2EHPA is a powerful synergistic reagent with calixarene for adenine extraction. It was demonstrated that one nucleobases forms a stable complex with one calixarene and two D2EHPA molecules, based on Job's method. Adenine was recovered from the organic phase by contacting with a fresh acidic solution.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Filtration and Separation