Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic golgi fragments in vitro

Catherine Rabouille, Hisao Kondo, Richard Newman, Norman Hui, Paul Freemont, Graham Warren

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217 Citations (Scopus)

Abstract

A cell-free system that mimics the reassembly of Golgi stacks at the end of mitosis requires two ATPases, NSF and p97, to rebuild Golgi cisternae. Morphological studies now show that α-SNAP, a component of the NSF pathway, can inhibit the p97 pathway, whereas p47, a component of the p97 pathway, can inhibit the NSF pathway. Anti-syntaxin 5 antibodies and a soluble, recombinant syntaxin 5 inhibited both pathways, suggesting that this t-SNARE is a common component. Biochemical studies confirmed this, showing that p47 binds directly to syntaxin 5 and competes for binding with α-SNAP. p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to α-SNAP, which mediates the binding of NSF.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalCell
Volume92
Issue number5
DOIs
Publication statusPublished - Mar 6 1998
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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