Synthesis and characterization of vinyl polymer-grafted DNA and its AB-type block conjugate with native DNA fragment

Daisuke Umeno, Masami Kiji, Masaharu Murata, Mizuo Maeda

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    We have synthesized psoralen-terminated poly(N-isopropylacrylamide)(1) and poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide)(2), both of which show temperature-responsive properties and DNA-binding ability. These polymers were grafted on plasmid double stranded DNA through a photochemical reaction of their terminal psoralen groups. The double stranded DNA grafted with the vinyl polymers turned out to lose the function as templates for RNA synthesis, while it showed characteristic solution property derived from the vinyl polymers. Ligation reaction between the polymer-grafted DNA and native DNA resulted in the AB-type block conjugate with the polymer-grafted DNA as A-segment and native DNA as B-segment. It was found that the block conjugate which have two different functional domains retains two functions independently; B-domain of the block conjugate worked as a template for RNA synthesis, while A-domain responded to temperature and made the conjugate precipitate. In this way, one can construct tailor-made and precisely-regulated structures with multiple functions at his own will by using recombinant DNA techniques.

    Original languageEnglish
    Pages (from-to)1109-1114
    Number of pages6
    JournalPolymer Journal
    Volume31
    Issue number11 pt 2
    Publication statusPublished - Dec 1 1999

    Fingerprint

    Polymers
    DNA
    Ficusin
    RNA
    Recombinant DNA
    Photochemical reactions
    Precipitates
    Plasmids
    Temperature

    All Science Journal Classification (ASJC) codes

    • Polymers and Plastics
    • Materials Chemistry

    Cite this

    Synthesis and characterization of vinyl polymer-grafted DNA and its AB-type block conjugate with native DNA fragment. / Umeno, Daisuke; Kiji, Masami; Murata, Masaharu; Maeda, Mizuo.

    In: Polymer Journal, Vol. 31, No. 11 pt 2, 01.12.1999, p. 1109-1114.

    Research output: Contribution to journalArticle

    Umeno, D, Kiji, M, Murata, M & Maeda, M 1999, 'Synthesis and characterization of vinyl polymer-grafted DNA and its AB-type block conjugate with native DNA fragment', Polymer Journal, vol. 31, no. 11 pt 2, pp. 1109-1114.
    Umeno, Daisuke ; Kiji, Masami ; Murata, Masaharu ; Maeda, Mizuo. / Synthesis and characterization of vinyl polymer-grafted DNA and its AB-type block conjugate with native DNA fragment. In: Polymer Journal. 1999 ; Vol. 31, No. 11 pt 2. pp. 1109-1114.
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