Synthesis and DNA cleaving activity of water-soluble non-conjugated thienyl tetraynes

Kohei Torikai, Yoichi Otsuka, Makoto Nishimura, Megumi Sumida, Tomoji Kawai, Kiyotoshi Sekiguchi, Ikuo Ueda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Water-soluble non-conjugated thienyl tetraynes (3-6) were synthesized and their DNA cleaving activity was evaluated using electrophoresis, atomic force microscopy (AFM) and Escherichia coli (E. coli) transformation techniques. The amino-functionalized compound 4 was shown to possess an activity to cleave plasmid DNA by both electrophoresis and E. coli transformation techniques. AFM also showed a cleavage of the circular DNA into a linear form with a formation of burst-star-shaped architectures, which were envisaged to be cross-linked DNA oligomers.

Original languageEnglish
Pages (from-to)5441-5451
Number of pages11
JournalBioorganic and Medicinal Chemistry
Volume16
Issue number10
DOIs
Publication statusPublished - May 15 2008
Externally publishedYes

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Atomic Force Microscopy
Electrophoresis
Escherichia coli
Water
Atomic force microscopy
DNA
Circular DNA
Oligomers
Stars
Plasmids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Synthesis and DNA cleaving activity of water-soluble non-conjugated thienyl tetraynes. / Torikai, Kohei; Otsuka, Yoichi; Nishimura, Makoto; Sumida, Megumi; Kawai, Tomoji; Sekiguchi, Kiyotoshi; Ueda, Ikuo.

In: Bioorganic and Medicinal Chemistry, Vol. 16, No. 10, 15.05.2008, p. 5441-5451.

Research output: Contribution to journalArticle

Torikai, K, Otsuka, Y, Nishimura, M, Sumida, M, Kawai, T, Sekiguchi, K & Ueda, I 2008, 'Synthesis and DNA cleaving activity of water-soluble non-conjugated thienyl tetraynes', Bioorganic and Medicinal Chemistry, vol. 16, no. 10, pp. 5441-5451. https://doi.org/10.1016/j.bmc.2008.04.009
Torikai, Kohei ; Otsuka, Yoichi ; Nishimura, Makoto ; Sumida, Megumi ; Kawai, Tomoji ; Sekiguchi, Kiyotoshi ; Ueda, Ikuo. / Synthesis and DNA cleaving activity of water-soluble non-conjugated thienyl tetraynes. In: Bioorganic and Medicinal Chemistry. 2008 ; Vol. 16, No. 10. pp. 5441-5451.
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