Synthesis and in vitro cell compatibility of α-tricalcium phosphate-based apatite cement containing tricalcium silicate

L. J. Cardenas, A. Takeuchi, Kanji Tsuru, S. Matsuya, Kunio Ishikawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The presence of silicate in artificial bone graft material is known to be effective in increasing bone formation rate. We previously reported the basic setting properties of α-tricalcium phosphate (α-Ca 3PO4; α-TCP) based apatite cement (AC) with various amount of tricalcium silicate (Ca3SiO5; alite) addition using sodium dihydrogen phosphate (NaH2PO4) as the liquid phase. In this study, in vitro biological compatibility of pure α-TCP and 2.5-10.0 wt% alite added to α-TCP based AC is investigated in terms of cell attachment, proliferation and differentiation. Set ACs aged for 168 hrs were used for cell studies with bone marrow cells from tibia of 4 week-old, male, SD rat. Initial cell attachment was observed after 7 hrs; cell proliferation was observed after 3, 5, and 7 days. Cell differentiation (alkaline phosphatase activity) was observed after 3, 6, and 9 days of incubation. Cell attachment of alite added ACs were almost the same as sintered hydroxyapatite (HAp) and alite-free AC. Cell proliferation at 2.5-7.5 wt% alite added ACs were significantly higher at day 5 and 7 (p<0.05). Alkaline phosphatase activity revealed that 2.5 wt% alite addition accelerated the osteogenic cell activity at day 6 and 9 (p<0.05) whereas too much alite addition resulted in the decrease of osteogenic cell activity. In conclusion, we found that α-TCP based AC containing appropriate amount of alite could be an improved AC since it shows suitable setting time, higher mechanical strength and possible higher osteoconductivity.

Original languageEnglish
Title of host publicationThe Third International Conference on the Development of Biomedical Engineering in Vietnam
Pages207-210
Number of pages4
DOIs
Publication statusPublished - May 21 2010
Event3rd International Conference on the Development of Biomedical Engineering in Vietnam - Ho Chi Minh City, Viet Nam
Duration: Jan 11 2010Jan 14 2010

Publication series

NameIFMBE Proceedings
Volume27
ISSN (Print)1680-0737

Other

Other3rd International Conference on the Development of Biomedical Engineering in Vietnam
CountryViet Nam
CityHo Chi Minh City
Period1/11/101/14/10

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Cite this

Cardenas, L. J., Takeuchi, A., Tsuru, K., Matsuya, S., & Ishikawa, K. (2010). Synthesis and in vitro cell compatibility of α-tricalcium phosphate-based apatite cement containing tricalcium silicate. In The Third International Conference on the Development of Biomedical Engineering in Vietnam (pp. 207-210). (IFMBE Proceedings; Vol. 27). https://doi.org/10.1007/978-3-642-12020-6-52