Synthesis and intracellular transportation of type i procollagen during functional differentiation of odontoblasts

Shigehisa Sato, Masahiro Tsuchiya, Ken Ichiro Komaki, Shin Ichiro Kusunoki, Shinobu Tsuchiya, Naoto Haruyama, Ichiro Takahashi, Yasuyuki Sasano, Makoto Watanabe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The expression of type I collagen, the most component of dentin extracellular matrix proteins (ECMs) in odontoblast is correlated with the activity of dentin formation. Since odontoblast possesses a distinct cellular process for protein transport into the dentinal tubule, it is important to examine the intracellular protein localization. However, a study focusing on odontoblast processes has not been performed. Type I collagen is synthesized as procollagen, which is immediately converted to collagen upon secretion. After characterization of antiserum to rat type I procollagen, we investigated the intracellular localization of type I procollagen in odontoblasts during and after dentinogenesis, using immunohistochemistry and in situ hybridization. The level of mRNA expression decreased during dentinogenesis, whereas the intracellular localization of type I procollagen in odontoblast processes become more distinct. The percentage of dentinal tubules with type I procollagen increased significantly with aging. Odontoblasts in pulp horn, in particular, showed moderate expression of type I procollagen after dentinogenesis. Since loss of occlusion also caused a significant decrease in type I procollagen, we concluded that occlusal stimulation activated type I procollagen synthesis in odontoblasts. We also suggest that analysis of intracellular transport of type I procollagen via odontoblast processes may be a new approach to evaluation of odontoblast function.

Original languageEnglish
Pages (from-to)583-591
Number of pages9
JournalHistochemistry and Cell Biology
Volume131
Issue number5
DOIs
Publication statusPublished - May 1 2009

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Odontoblasts
Procollagen
Collagen Type I
Dentinogenesis
Dentin
Extracellular Matrix Proteins
Protein Transport
Horns
In Situ Hybridization
Immune Sera
Collagen
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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Synthesis and intracellular transportation of type i procollagen during functional differentiation of odontoblasts. / Sato, Shigehisa; Tsuchiya, Masahiro; Komaki, Ken Ichiro; Kusunoki, Shin Ichiro; Tsuchiya, Shinobu; Haruyama, Naoto; Takahashi, Ichiro; Sasano, Yasuyuki; Watanabe, Makoto.

In: Histochemistry and Cell Biology, Vol. 131, No. 5, 01.05.2009, p. 583-591.

Research output: Contribution to journalArticle

Sato, Shigehisa ; Tsuchiya, Masahiro ; Komaki, Ken Ichiro ; Kusunoki, Shin Ichiro ; Tsuchiya, Shinobu ; Haruyama, Naoto ; Takahashi, Ichiro ; Sasano, Yasuyuki ; Watanabe, Makoto. / Synthesis and intracellular transportation of type i procollagen during functional differentiation of odontoblasts. In: Histochemistry and Cell Biology. 2009 ; Vol. 131, No. 5. pp. 583-591.
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