Synthesis and Thrombogenicity Evaluation of Poly(3-methoxypropionic acid vinyl ester): A Candidate for Blood-Compatible Polymers

Kazuhiro Sato, Shingo Kobayashi, Asuka Sekishita, Miyuki Wakui, Masaru Tanaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple two-step reaction, and then the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the nonthrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and enzyme-linked immunosorbent assay. The adsorption and denaturation of the plasma proteins were inhibited on the PMePVE; thus, PMePVE exhibited blood compatibility. A distinctive hydration water structure in the nonthrombogenic polymer, intermediate water (IW), was observed in the hydrated PMePVE by differential scanning calorimetry analysis. The nonthrombogenicity of PMePVE is considered to be brought about by the presence of IW.

Original languageEnglish
Pages (from-to)1609-1616
Number of pages8
JournalBiomacromolecules
Volume18
Issue number5
DOIs
Publication statusPublished - May 8 2017

Fingerprint

Esters
Polymers
Blood
Derivatives
Acids
Platelets
Water
Blood Proteins
Assays
Adhesion
Proteins
Plasmas
Adsorption
Immunosorbents
Denaturation
vinyl acetate
Free radical polymerization
Hydration
Fibrinogen
Differential scanning calorimetry

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Synthesis and Thrombogenicity Evaluation of Poly(3-methoxypropionic acid vinyl ester) : A Candidate for Blood-Compatible Polymers. / Sato, Kazuhiro; Kobayashi, Shingo; Sekishita, Asuka; Wakui, Miyuki; Tanaka, Masaru.

In: Biomacromolecules, Vol. 18, No. 5, 08.05.2017, p. 1609-1616.

Research output: Contribution to journalArticle

Sato, Kazuhiro ; Kobayashi, Shingo ; Sekishita, Asuka ; Wakui, Miyuki ; Tanaka, Masaru. / Synthesis and Thrombogenicity Evaluation of Poly(3-methoxypropionic acid vinyl ester) : A Candidate for Blood-Compatible Polymers. In: Biomacromolecules. 2017 ; Vol. 18, No. 5. pp. 1609-1616.
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