TY - JOUR
T1 - Synthesis and Thrombogenicity Evaluation of Poly(3-methoxypropionic acid vinyl ester)
T2 - A Candidate for Blood-Compatible Polymers
AU - Sato, Kazuhiro
AU - Kobayashi, Shingo
AU - Sekishita, Asuka
AU - Wakui, Miyuki
AU - Tanaka, Masaru
N1 - Funding Information:
S.K. gratefully acknowledges financial support from JSPS KAKENHI (No. 15K05512) of Japan Society of the Promotion of Science (JSPS). K.S. acknowledges Grant-in-Aid for JSPS Fellows (No. 15J08130). M.T. acknowledges financial support from the Funding Program for Next-Generation World-Leading Researchers (NEXT Program) of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan and the Center of Innovation Program from the Japan Science and Technology Agency (JST). This work was partially supported by JST ERATO (No. JPMJER1105).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/5/8
Y1 - 2017/5/8
N2 - A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple two-step reaction, and then the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the nonthrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and enzyme-linked immunosorbent assay. The adsorption and denaturation of the plasma proteins were inhibited on the PMePVE; thus, PMePVE exhibited blood compatibility. A distinctive hydration water structure in the nonthrombogenic polymer, intermediate water (IW), was observed in the hydrated PMePVE by differential scanning calorimetry analysis. The nonthrombogenicity of PMePVE is considered to be brought about by the presence of IW.
AB - A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple two-step reaction, and then the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the nonthrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and enzyme-linked immunosorbent assay. The adsorption and denaturation of the plasma proteins were inhibited on the PMePVE; thus, PMePVE exhibited blood compatibility. A distinctive hydration water structure in the nonthrombogenic polymer, intermediate water (IW), was observed in the hydrated PMePVE by differential scanning calorimetry analysis. The nonthrombogenicity of PMePVE is considered to be brought about by the presence of IW.
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U2 - 10.1021/acs.biomac.7b00221
DO - 10.1021/acs.biomac.7b00221
M3 - Article
C2 - 28391697
AN - SCOPUS:85019073246
SN - 1525-7797
VL - 18
SP - 1609
EP - 1616
JO - Biomacromolecules
JF - Biomacromolecules
IS - 5
ER -