Synthesis of β-D-Fucosylglucose by β-D-Glucosidase I of Bifidobacterium breve clb and Assimilation by Bifidobacteria

Kenji Sakai, Takashi Tachiki, Hidehiko Kumagai, Tatsurokuro Tochikura

Research output: Contribution to journalArticle

Abstract

The p-nitrophenol-releasing activity, from/Miitrophenyl (p-NP) β-d-fucoside, of β-d-glucosidase I from Bifidobacterium breve clb was enhanced by the addition of many kinds of sugars and alcohols, suggesting the occurrence of a β-d-fucosyl transferring reaction. The enhancement on glucose addition was dependent on the reaction pH, and the concentrations of p-NP β-d-fucoside and glucose, and the activity reached 430 % when 100 mM glucose was added to the mixture containing 20 mM p-NP β-d-fucoside at pH 4.5. A mixture of transfer products was separated from the other constituents by activated charcoal column chromatography. Further purification of the mixture by paper and thinlayer chromatographies gave four oligosaccharides, which were identified as β-d-fucosylglucoses with β 1→2, β 1→3, β 1→4 and β 1→6, linkages from the absorption spectra obtained with the phenolsulfuric acid method, and the results of acid and enzymatic hydrolyses, and methylation analyses. The mixture of oligosaccharides was well assimilated by many bifidobacteria but not by any other intestinal bacteria tested.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalAgricultural and Biological Chemistry
Volume53
Issue number2
DOIs
Publication statusPublished - Jan 1 1989

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Bifidobacterium breve
glucosidases
Bifidobacterium
assimilation (physiology)
Oligosaccharides
Glucose
synthesis
oligosaccharides
glucose
Sugar Alcohols
Paper Chromatography
chromatography
Acids
Charcoal
p-nitrophenol
Methylation
Column chromatography
Enzymatic hydrolysis
Chromatography
acid hydrolysis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Synthesis of β-D-Fucosylglucose by β-D-Glucosidase I of Bifidobacterium breve clb and Assimilation by Bifidobacteria. / Sakai, Kenji; Tachiki, Takashi; Kumagai, Hidehiko; Tochikura, Tatsurokuro.

In: Agricultural and Biological Chemistry, Vol. 53, No. 2, 01.01.1989, p. 313-318.

Research output: Contribution to journalArticle

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